Recombinant Human Proteasome Subunit Alpha Type-7 (PSMA7) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-08511P
Greater than 90% as determined by SDS-PAGE.
Recombinant Human Proteasome Subunit Alpha Type-7 (PSMA7) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-08511P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | Recombinant Human Proteasome Subunit Alpha Type-7 (PSMA7) Protein (GST) is produced by our E.coli expression system. This is a protein fragment. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Uniprotkb | O14818 |
| Target Symbol | PSMA7 |
| Synonyms | C6; HSPC; MGC3755; OTTHUMP00000031449; OTTHUMP00000031450; OTTHUMP00000031453; Proteasome (prosome macropain) subunit alpha type 7; Proteasome alpha 7 subunit; Proteasome subunit alpha 4; Proteasome subunit alpha type 7; Proteasome subunit alpha type-7; Proteasome subunit RC6 1; Proteasome subunit RC6-1; Proteasome subunit XAPC7; PSA7_HUMAN; PSMA 7; PSMA7; RC6 1; XAPC7 |
| Species | Homo sapiens (Human) |
| Expression System | E.coli |
| Tag | N-GST |
| Target Protein Sequence | RAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEI |
| Expression Range | 5-232aa |
| Protein Length | Partial |
| Mol. Weight | 52.4kDa |
| Research Area | Immunology |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
| Target Function | Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response. |
| Subcellular Location | Cytoplasm. Nucleus. |
| Protein Families | Peptidase T1A family |
| Database References |
Gene Functions References
- salivary exosomal PSMA7 was present at high levels in salivary exosomes from subjects with Inflammatory bowel disease. It can be a very promising biomarker PMID: 28523434
- Studies have found significant associations of the treatment response with the 26S proteasome non-ATPase subunit 9 (PSMD9), proteasome alpha type 7 subunit (PSMA7) and PSMD13 genes. PMID: 26624926
- c-Abl regulates proteasome abundance by controlling the ubiquitin-proteasomal degradation of PSMA7 subunit PMID: 25620702
- PSMA7 functions partially through downregulation NOD1. PMID: 23839082
- PSMA7 inhibits the proliferation, tumorigenicity and invasion of A549 cells in vitro PMID: 22584585
- High expression of PSMA7 was significantly correlated with liver metastasis. PMID: 19062718
- The present study demonstrates that c-Abl and Arg (abl-related gene) tyrosine kinases associate with and phosphorylate the proteasome PSMA7 (alpha4) subunit at Tyr-153. PMID: 16678104
- PSMA7 may play an important role in colorectal cancer progression. PMID: 18202793
- cell surface binding of EMAP-II results in its internalization into the cytoplasmic compartment where it interacts with PSMA7, a component of the proteasome degradation pathway. This interaction increases HIF-1alpha degradation under hypoxic conditions. PMID: 19362550
