Recombinant E.Coli Aquaporin Z (AQPZ) Protein (His), Active

Name: Recombinant E.Coli Aquaporin Z (AQPZ) Protein (His), Active
Brand: Beta LifeScience
SKU: BLC-05643P
Price: 3154.4 USD
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Activity Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC 50 of E.coli ytfE protein is 197.90-259.70 μg/ml.

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant E.Coli Aquaporin Z (AQPZ) Protein (His), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Activity	Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC50 of E.coli ytfE protein is 197.90-259.70 μg/ml.
Uniprotkb	P60844
Target Symbol	AQPZ
Synonyms	aqpZ; bniP; b0875; JW0859; Aquaporin Z; Bacterial nodulin-like intrinsic protein
Species	Escherichia coli (strain K12)
Expression System	in vitro E.coli expression system
Tag	N-6His
Target Protein Sequence	MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD
Expression Range	1-231aa
Protein Length	Full Length
Mol. Weight	27.7kDa
Research Area	Microbiology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.
Subcellular Location	Cell inner membrane; Multi-pass membrane protein.
Protein Families	MIP/aquaporin (TC 1.A.8) family
Database References	KEGG: ecj:JW0859 STRING: 316385.ECDH10B_0945

Gene Functions References

Induction of an immune response against AqpZ or its homologous regions can also trigger an autoimmune reaction against human Aqp4 and inflammation of the central nervous system. PMID: 23008451
Data show that the open and closed states of AqpZ directly relate to the side chain conformation of residue R189. PMID: 21315687
the 2.5 A resolution structure of AqpZ suggests aquaporin selectivity results both from a steric mechanism due to pore size and from specific amino acid substitutions that regulate the preference for a hydrophobic or hydrophilic substrate PMID: 14691544
the difference in Arg-189 displacements is correlated with a strong electron density between first transmembrane helices of two open channels; the observed Arg-189 conformations are stabilized by asymmetrical subunit interactions in tAqpZ PMID: 16239219
analysis of of Escherichia coli aquaporin AqpZ and GlpF single-channel water permeabilities PMID: 16399837
biochemical analysis of permeability and solute transport characteristics of amphiphilic triblock-polymer vesicles containing the bacterial water-channel protein Aquaporin Z PMID: 18077364

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.