Recombinant Bacillus Subtilis Uncharacterized Protein Yuab (YUAB) Protein (His-SUMO), Active
Beta LifeScience
SKU/CAT #: BLC-05649P
Recombinant Bacillus Subtilis Uncharacterized Protein Yuab (YUAB) Protein (His-SUMO), Active
Beta LifeScience
SKU/CAT #: BLC-05649P
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Bacillus Subtilis Uncharacterized Protein Yuab (YUAB) Protein (His-SUMO), Active is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Activity | Measured by its binding ability in a functional ELISA. Immobilized yuaB at 5 μg/ml can bind human HP_0175 with a linear range of 31.25-600.00 ng/ml. |
Uniprotkb | P71014 |
Target Symbol | YUAB |
Synonyms | bslA; yuaB; BSU31080; Biofilm-surface layer protein A; ORF-1 |
Species | Bacillus subtilis (strain 168) |
Expression System | in vitro E.coli expression system |
Tag | N-6His-SUMO |
Target Protein Sequence | MKRKLLSSLAISALSLGLLVSAPTASFAAESTSTKAHTESTMRTQSTASLFATITGASKTEWSFSDIELTYRPNTLLSLGVMEFTLPSGFTANTKDTLNGNALRTTQILNNGKTVRVPLALDLLGAGEFKLKLNNKTLPAAGTYTFRAENKSLSIGNKFYAEASIDVAKRSTPPTQPCGCN |
Expression Range | 1-181aa |
Protein Length | Full Length |
Mol. Weight | 35.3kDa |
Research Area | Others |
Form | Lyophilized powder |
Buffer | Lyophilized from 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Involved in biofilm formation. Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm. The layer is stable and capable of resistance to high mechanical force compression. Required for complex colony architecture. May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix. |
Subcellular Location | Secreted. Secreted, cell wall. |
Database References | KEGG: bsu:BSU31080 STRING: 224308.Bsubs1_010100016901 |
Gene Functions References
- Taken together, these findings identify YuaB as a protein that plays a novel role during biofilm formation. PMID: 21742882