Biotinylated Recombinant Rat Serum Albumin (ALB) Protein (MBP&His-Avi)

Name: Biotinylated Recombinant Rat Serum Albumin (ALB) Protein (MBP&His-Avi)
Brand: Beta LifeScience
SKU: BLC-06708P
Price: 946.4 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Avi-tagged proteins, Biotinylated proteins, Featured biotinylated protein molecules, Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Biotinylated Recombinant Rat Serum Albumin (ALB) Protein (MBP&His-Avi) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P02770
Target Symbol	ALB
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-MBP&C-6His-Avi
Target Protein Sequence	EAHKSEIAHRFKDLGEQHFKGLVLIAFSQYLQKCPYEEHIKLVQEVTDFAKTCVADENAENCDKSIHTLFGDKLCAIPKLRDNYGELADCCAKQEPERNECFLQHKDDNPNLPPFQRPEAEAMCTSFQENPTSFLGHYLHEVARRHPYFYAPELLYYAEKYNEVLTQCCTESDKAACLTPKLDAVKEKALVAAVRQRMKCSSMQRFGERAFKAWAVARMSQRFPNAEFAEITKLATDVTKINKECCHGDLLECADDRAELAKYMCENQATISSKLQACCDKPVLQKSQCLAEIEHDNIPADLPSIAADFVEDKEVCKNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEGDPPACYGTVLAEFQPLVEEPKNLVKTNCELYEKLGEYGFQNAVLVRYTQKAPQVSTPTLVEAARNLGRVGTKCCTLPEAQRLPCVEDYLSAILNRLCVLHEKTPVSEKVTKCCSGSLVERRPCFSALTVDETYVPKEFKAETFTFHSDICTLPDKEKQIKKQTALAELVKHKPKATEDQLKTVMGDFAQFVDKCCKAADKDNCFATEGPNLVARSKEALA
Expression Range	25-608aa
Protein Length	Full Length of Mature Protein
Mol. Weight	113.7 kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
Subcellular Location	Secreted.
Protein Families	ALB/AFP/VDB family
Database References	KEGG: rno:24186 STRING: 10116.ENSRNOP00000003921 UniGene: Rn.202968
Tissue Specificity	Plasma.

Gene Functions References

Tubular Expression and Urinary Excretion of MMP-9 Protein Were Increased in Association with Albumin Overload in the Diabetic Kidneys. PMID: 28805677
albumin immunoreactivity was newly observed in microglia in the hippocampal CA1 region following 5 min of transient cerebral ischemia. These results indicate that transient ischemia-induced albumin expression in microglia might be associated with ischemia-induced 'delayed neuronal death' in the hippocampal CA1 pyramidal neurons. PMID: 28586018
significant structural changes occur to albumin with glycation, particularly in the FcRn-binding region, which could explain the reduced affinity to FcRn PMID: 26887834
Physiological Levels of Pentraxin 3 and Albumin Attenuate Vascular Endothelial Cell Damage Induced by Histone H3 PMID: 26806786
Megalin/cubilin and lysosome rupture is involved in albumin-triggered tubular injury and tubulointerstitial inflammation. PMID: 26025362
Both insulin and high glucose concentrations enhance the permeability of podocytes to albumin by stimulating oxygen free radical production, primarily by NAD(P)H oxidase-4 (NOX4), and by activating protein kinase G, isoform Ialpha. PMID: 25888796
Overall, rats with albumin deficiency display enhanced glucose tolerance, insulin secretion and gluconeogenic flux. PMID: 22198013
albumin prevents 6-hydroxydopamine-induced loss of tyrosine hydroxylase PMID: 22815976
Data indicate that transcription of Alb is regulated by a complicated system of distant enhancers. PMID: 21858039
This pioneering study determined a higher level of ischemia-modified albumin in a testicular torsion model. PMID: 20452583
Forced expression of PPAR-gamma or C/EBP-alpha in activated pancreatic stellate cells induced albumin expression, thereby reverting cells to the quiescent phenotype. PMID: 19932685
Catalysis of S-nitrosothiols formation by serum albumin: the mechanism and implication in vascular control PMID: 11983891
This study suggests that Aroclor 1254 induces elevated albumin serum levels indirectly through induction of liver-enriched transcription factors, which regulate albumin gene expression PMID: 12051991
an accelerated plasma efflux of albumin contributes to hypoalbuminemia only during the early period of sepsis PMID: 12571074
albumin affects glucose metabolism by impairing insulin-induced insulin receptor substrate (IRS) signaling through a protein kinase C alpha-mediated mechanism PMID: 12970360
Kinetics of albumin production during rat liver development. PMID: 17879097

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.