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Uracil-DNA Glycosylase (UDG)

Cat No.: ENY-105
Description
description E.coli Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases). It releases uracil from ss- or ds-DNA and is applicable to eliminates PCR carry-over contamination.
source An E.coli strain that carries the UDG gene from E. coli .
applications Eliminates PCR carry-over contamination
size 1000 U / 5000 U
concentration 5,000 U/ml
components UDG (5,000 U/ml); 10X UDG Reaction Buffer
unit definition One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50 µl reaction containing 0.2 µg DNA (104-105 cpm/µg) in 30 minutes at 37°C.
usage For Research Use Only
storage Storage Conditions: 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 mg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C. Store the Uracil-DNA Glycosylase (UDG) at -20°C. Please avoid repeated freeze-thaw cycles.
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