|synonym||Tn5; Tn5 Transposase; Transposase (Tnp) Tn5; Dimer_Tnp_Tn5|
|background||Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes. Bacterial Tn5 transposase promotes the transposition of the Tn5 transposon. Tn5 transposase integrates DNA fragments at a specific site in the nucleosomal DNA. In the ATAC-seq method, Tn5 transposase has been employed to insert an adaptor DNA fragment in the nucleosome-free DNA regions and linker DNA regions between nucleosomes.|
|description||Magic™ Tn5 Transposase is a naked and hyperactive form of Tn5 transposase. Our Magic Tn5 Transposase can recognize inside, outside and mosaic end sequences of Tn5 transposon.|
|applications||• Library construction for second-generation sequencing.
• In vitro transgenic experiment.
|components||a. Tn5 Transposase(1 U/μl)
b. 5× LM Buffer
c. 10×TPS Buffer
|unit definition||One unit Tn5 Transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition seqence in 1 hour at 37°C.|
|storage buffer||50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol (V/V)|
|usage||For Research Use Only|
|storage||The Tn5 Transposase is shipped with dry ice. Store Tn5 Transposase at -20℃. Avoid repeated freeze-thaw cycles.|