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Tn5 Transposase

Cat No.: TN5-BL01
Transposase tn5
Description QC Data
synonym Tn5; Tn5 Transposase; Transposase (Tnp) Tn5; Dimer_Tnp_Tn5
background Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes. Bacterial Tn5 transposase promotes the transposition of the Tn5 transposon. Tn5 transposase integrates DNA fragments at a specific site in the nucleosomal DNA. In the ATAC-seq method, Tn5 transposase has been employed to insert an adaptor DNA fragment in the nucleosome-free DNA regions and linker DNA regions between nucleosomes.
description Magic™ Tn5 Transposase is a naked and hyperactive form of Tn5 transposase. Our Magic Tn5 Transposase can recognize inside, outside and mosaic end sequences of Tn5 transposon.
source E.coli
applications • Library construction for second-generation sequencing.
• In vitro transgenic experiment.
• ATAC-seq
size 25U
concentration 1 U/μl.
components a. Tn5 Transposase(1 U/μl)
b. 5× LM Buffer
c. 10×TPS Buffer
unit definition One unit Tn5 Transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition seqence in 1 hour at 37°C.
storage buffer 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol (V/V)
usage For Research Use Only
storage The Tn5 Transposase is shipped with dry ice. Store Tn5 Transposase at -20℃. Avoid repeated freeze-thaw cycles.
This product has passed the following quality control assays: functional absence of exonuclease and RNase activities.  Absence of exonucleases activity: Hind III fragments (1 µg) of lambda DNA is incubated with our Tn5 Transposase (20U) in  50µl test buffer, overlaid with paraffin oil, at 37°C for 4 hours. The electrophoretic bands show no obvious alteration.  Absence of RNase activity: E.coli rRNA (250 ng) is incubated with our Tn5 Transposase (100U) in 50µl test buffer, overlaid  with paraffin oil, at 37°C for 2 hours. The degradation of rRNA is less than 1%.

 
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