Tn5 Transposase

Name: Tn5 Transposase
Brand: BetaLifeSci
SKU: TN5-BL01
Price: 649.0 USD
Availability: InStock

Collections: Featured enzyme molecules, Tool enzymes & other reagents

Price

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Product Overview

Synonym	Tn5; Tn5 Transposase; Transposase (Tnp) Tn5; Dimer_Tnp_Tn5
Background	Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes. Bacterial Tn5 transposase promotes the transposition of the Tn5 transposon. Tn5 transposase integrates DNA fragments at a specific site in the nucleosomal DNA. In the ATAC-seq method, Tn5 transposase has been employed to insert an adaptor DNA fragment in the nucleosome-free DNA regions and linker DNA regions between nucleosomes.
Description	Magic™ Tn5 Transposase is a naked and hyperactive form of Tn5 transposase. Our Magic Tn5 Transposase can recognize inside, outside and mosaic end sequences of Tn5 transposon.
Source	E.coli
Applications	Library construction for second-generation sequencing. In vitro transgenic experiment. ATAC-seq
Size	25U
Concentration	1 U/μl.
Components	a. Tn5 Transposase(1 U/μl) b. 5 * LM Buffer c. 10 * TPS Buffer
Unit Definition	One unit Tn5 Transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition sequence in 1 hour at 37°C.
Storage Buffer	50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol (V/V)
Usage	For Research Use Only
Storage	The Tn5 Transposase is shipped with dry ice. Store Tn5 Transposase at -20°C. Avoid repeated freeze-thaw cycles.

Citations

Title: Simultaneous trimodal single-cell measurement of transcripts, epitopes, and chromatin accessibility using TEA-seq
Author: Elliott Swanson, Cara Lord, Julian Reading, Alexander T Heubeck, Palak C Genge, Zachary Thomson, Morgan DA Weiss, Xiao-jun Li, Adam K Savage, Richard R Green, Troy R Torgerson, Thomas F Bumol, Lucas T Graybuck, Peter J Skene
Year: 2021
Journal: eLife 10:e63632
doi: https://doi.org/10.7554/eLife.63632

Title: TEA-seq: a trimodal assay for integrated single cell measurement of transcription, epitopes, and chromatin accessibility
Author: Elliott Swanson, Cara Lord, Julian Reading, Alexander T. Heubeck, Adam K. Savage, Richard Green, Xiao-jun Li, Troy R. Torgerson, Thomas F. Bumol, Lucas T. Graybuck, Peter J. Skene
Year: 2020
Journal: bioRxiv 2020.09.04.283887
doi: https://doi.org/10.1101/2020.09.04.283887

Cited Paper Summary and Product Application Summary

View all

Tn5

Swanson, Elliott et al. “Simultaneous trimodal single-cell measurement of transcripts, epitopes, and chromatin accessibility using TEA-seq.” eLife vol. 10 e63632. 9 Apr. 2021, doi:10.7554/eLife.63632

May 25, 2023

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.