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T4 Polynucleotide Kinase

Cat No.: ENY-103
Description
description T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates. T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5´-phosphates to oligonucleotides to allow subsequent ligation and removal of 3´-phosphoryl groups.
source A E. coli strain that carries the cloned T4 Polynucleotide Kinase gene.
applications Phosphorylation (Kinase)
size 250 U / 500 U / 2500 U
concentration 10,000 U/ml
components T4 Polynucleotide Kinase (10,000 U/ml);10X T4 PNK Reaction Buffer
unit definition One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X T4 PNK Reaction Buffer with 66 µM [γ-32P] ATP (5 x 106 cpm/µmol) and 0.26 mM 5´-hydroxyl-terminated salmon sperm DNA.
usage For Research Use Only
storage Store the T4 Polynucleotide Kinase at -20°C. Please avoid repeated freeze-thaw cycles.
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