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T4 DNA Polymerase

Cat No.: ENY-106
description T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of template and primer. This enzyme has a 3'→5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5'→3' exonuclease function. It is applicable to 3´ overhang removal to form blunt ends, 5´ overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis. Theoretical protein molecular weight is 104000 daltons. Error Rate: ~ 1x10-6 bases
source Purified from a strain of E. coli that carries the T4 DNA Polymerase gene.
applications Blunting, PCR, Polymerases for DNA Manipulation
size 200 U / 1000 U
concentration 5,000 U/ml
components T4 DNA Polymerase (5,000 U/ml); 10X ABuffer B
unit definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
usage For Research Use Only
storage Storage Conditions: 100 mM KPO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C. Store the T4 DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles.
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