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RNase Inhibitor, Mammalian

Cat No.: ENY-126
description RNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 10^14.
source An E.coli strain that carries the Ribonuclease Inhibitor gene from Porcine.
applications Cell-Free Protein Expression, PCR, RT-PCR,cDNA synthesis,In vitro transcription/translation,Enzymatic RNA labeling reaction,Other applications where the integrity of RNA is important
size 2000 U / 10000 U / 20000 U
concentration 40,000 U/ml
components RNase Inhibitor, Mammalian (40,000 U/ml)
unit definition One unit is defined as the amount of RNase Inhibitor, Mammalian required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.
usage For Research Use Only
storage Store the RNase Inhibitor, Mammalian at -20°C. Please avoid repeated freeze-thaw cycles.
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