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Recombinant SARS coronavirus (SARS-CoV) (strain WH20) Plpro / papain-like protease (His Tag)

Cat No.: BLPSN-0980P
Description Related products
tag His
host species SARS
accession AAX16193.1
background The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses.
description A DNA sequence encoding the SARS coronavirus (SARS-CoV) (strain WH20) papain-like protease (PLpro) (AAX16193.1) (Glu1541-Tyr1859) was expressed in E.coli and fused with a His tag at the C-terminus.
source E.coli
predicted n terminal Glu 1541
AA sequence Glu1541-Tyr1859
molecular weight The recombinant SARS coronavirus (SARS-CoV) (strain WH20) papain-like protease (PLpro) consists of 319 amino acids and predicts a molecular mass of 35.8 kDa.
purity > 90% as determined by SDS-PAGE.
formulation The recombinant SARS coronavirus (SARS-CoV) (strain WH20) papain-like protease (PLpro) was lyophilized from sterile 50 mM Tris, 0.1 % Tween, pH 9.0.
stability The recombinant SARS coronavirus (SARS-CoV) (strain WH20) papain-like protease (PLpro) is stable for up to 1 year from date of receipt at -20℃ to -80℃.
usage For Research Use Only
storage Store the antigen under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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