Recombinant Rat SIRP alpha / SIRPA Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4265

Recombinant Rat SIRP alpha / SIRPA Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4265
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Tag His
Host Species Rat
Accession P97710
Synonym SIRPA Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; CD172 Antigen-Like Family Member A;Signal-Regulatory Protein Alpha-1; CD172a; SIRPA; MFR; SHPS1; SIRP
Background SIRPA is a type I transmembrane glycoprotein.It contains two Ig-like C1-type domains and one Ig-like V-type domain. Mouse SIRP alpha ECD shares 61%, 75%, 62%, 61%, and 59% aa sequence identity with human, rat, equine, bovine, and porcine SIRP alpha, respectively.SIRPA can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPA engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation.
Description A DNA sequence encoding the rat SIRPA (P97710) (Met1-Asn373) was produced with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Lys 32
AA Sequence Met1-Asn373
Molecular Weight The recombinant rat SIRPA comprises 353 amino acids and predicts a molecular mass of 39 kDa. The apparent molecular mass of the recombinant protein is approximately 63-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity Greater than 95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1.Measured by its binding ability in a functional ELISA. 2.Immobilized rat SIRPA-His at 10ug/mL (100uL/well) can bind biotinylated rat CD47-His, the EC50 of biotinylated rat CD47-His is 0.1-0.4ug/mL.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C
Usage For Research Use Only

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

Recently viewed