Recombinant Rat SIRP alpha / SIRPA Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4265
Recombinant Rat SIRP alpha / SIRPA Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4265
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Rat |
Accession | P97710 |
Synonym | SIRPA Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; CD172 Antigen-Like Family Member A;Signal-Regulatory Protein Alpha-1; CD172a; SIRPA; MFR; SHPS1; SIRP |
Background | SIRPA is a type I transmembrane glycoprotein.It contains two Ig-like C1-type domains and one Ig-like V-type domain. Mouse SIRP alpha ECD shares 61%, 75%, 62%, 61%, and 59% aa sequence identity with human, rat, equine, bovine, and porcine SIRP alpha, respectively.SIRPA can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPA engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation. |
Description | A DNA sequence encoding the rat SIRPA (P97710) (Met1-Asn373) was produced with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Lys 32 |
AA Sequence | Met1-Asn373 |
Molecular Weight | The recombinant rat SIRPA comprises 353 amino acids and predicts a molecular mass of 39 kDa. The apparent molecular mass of the recombinant protein is approximately 63-80 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Purity | Greater than 95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1.Measured by its binding ability in a functional ELISA. 2.Immobilized rat SIRPA-His at 10ug/mL (100uL/well) can bind biotinylated rat CD47-His, the EC50 of biotinylated rat CD47-His is 0.1-0.4ug/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C |
Usage | For Research Use Only |