Recombinant Rat CCL4 Protein (His Tag)

Collections: Chemokines and receptors, Cytokines and growth factors, Recombinant proteins

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Product Overview

Tag	His
Host Species	Rat
Accession	NP_446310.1
Synonym	CCL4L1, AT744.2, CCL4L, LAG-1, LAG1, SCYA4L, SCYA4L1, MIP-1-beta, SCYA4L2, C-C motif chemokine ligand 4 like 1
Background	Chemokine (C-C motif) ligands 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. CCL4, also known as Macrophage inflammatory protein-1beta (MIP-1beta) is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells.
Description	A DNA sequence encoding the rat Ccl4 (Ala24-Asn92) was expressed with a His tag at the C-terminus.
Source	Yeast
Predicted N Terminal	Ala 24
AA Sequence	Ala24-Asn92
Molecular Weight	The recombinant rat Ccl4 consists of 79 amino acids and predicts a molecular mass of 9.2 kDa.
Purity	>90% as determined by SDS-PAGE.
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Recombinant Rat CCL4 Protein (His Tag) was lyophilized from sterile 30 % CAN, 0.1 % TFA.
Stability	Recombinant Rat CCL4 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Monokine with inflammatory and chemokinetic properties.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	KEGG: rno:116637 STRING: 10116.ENSRNOP00000015199 UniGene: Rn.37880

Gene Functions References

Tong Luo Jiu Nao injection inhibits MIP-1beta expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation and inhibitors microglial cell proliferation. PMID: 22366434
Early and persistent up-regulation of CCL4/CCR5 signaling during epileptogenesis suggests that CCL4 signaling, rather than CCL2 signaling, could have a role in the epileptogenic process. PMID: 22353418
this study we present data on long term increase in CCL4 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
During experimental allergic encephalomyelitis, a strong activation of spinal perivascular macrophages is observed accompanied by a consistently increased expression of MIP-1 alpha. PMID: 12196270

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.