Recombinant Mouse PD-L2 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-3759

Recombinant Mouse PD-L2 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-3759
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Product Overview

Tag Fc
Host Species Mouse
Accession Q9WUL5
Synonym PD-L2 Programmed Cell Death 1 Ligand 2; PD-1 Ligand 2; PD-L2; PDCD1 Ligand 2; Programmed Death Ligand 2; Butyrophilin B7-DC; B7-DC; CD273; PDCD1LG2; B7DC; CD273; PDCD1L2; PDL2
Background Programmed Cell Death 1 Ligand 2 (PDCD1LG2) is a member of the BTN/MOG family. PDCD1LG2 contains one Ig-like C2-type domain and one Ig-like V-type domain. PDCD1LG2 is highly expressed in the heart, placenta, pancreas, lung and liver; it is weakly expressed in the spleen, lymph nodes, and thymus. PDCD1LG2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PDCD1LG2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Description A DNA sequence encoding the extracellular domain of mouse PDCD1LG2 (Q9WUL5) (Met 1-Arg 219) was fused with the Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Leu 20
AA Sequence Met 1-Arg 219
Molecular Weight The recombinant mouse PDCD1LG2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 441 amino acids and has a calculated molecular mass of 49.5 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 60 kDa in SDS-PAGE under reducing conditions.
Purity Greater than 95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured by its binding ability in a functional ELISA.2. Immobilized human PD1-His at 10 ug/mL (100 ul/well) can bind mouse PD-L2-Fc, The EC50 of mouse PD-L2-Fc is 0.95 ug/mL.
Formulation Lyophilized from sterile PBS, pH 7.4
Stability Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C
Usage For Research Use Only

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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