Recombinant Mouse IL-15RA Protein (C-Fc)

Collections: Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Interleukin-15 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Gly33-Lys205 is expressed with a human IgG1 Fc tag at the C-terminus.
Accession	Q60819
Synonym	Interleukin-15 receptor subunit alpha; Il15ra; sIL-15 receptor subunit alpha
Gene Background	Mouse interleukin-15 receptor subunit alpha, also known as Il15ra, is a high-affinity receptor for interleukin-15. Il15ra associates as a heterotrimer with the IL-2 receptor beta and gamma subunits (Common gamma chain, or gamma c) to initiate signal transduction. It can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Il15ra is expressed in special cells including a wide variety of Tand B cells and non-lymphoid cells. Human Il15ra shares 45% amino acid sequence homology with the mouse form of the receptor. Eight isoforms of IL-15 R alpha mRNA have been identified, resulting from alternative splicing events involving different exons.
Molecular Mass	45.5 KDa
Apmol Mass	60-90 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. In neutrophils, binds and activates kinase SYK in response to IL15 stimulation. In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner.
Subcellular Location	Membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Cell surface.; [Soluble interleukin-15 receptor subunit alpha]: Secreted, extracellular space.
Database References	KEGG: mmu:16169 STRING: 10090.ENSMUSP00000077878 UniGene: Mm.200196
Tissue Specificity	Widely expressed.

Gene Functions References

these data support that IL15RA plays a cell-autonomous role in osteoblast function and bone mineralization PMID: 28602725
These data provide additional evidence to implicate IL-15Ralpha as a regulator of skeletal muscle phenotypes through effects on the mitochondrion, and suggest these effects are driven by alterations to the mitochondrial proteome. PMID: 26458787
Spontaneous cage activity was not different and IL-15 protein levels were lower in male and female mIl15ra(fl/fl)/Cre(+) mice. PMID: 25505029
Despite being protected against diet induced obesity, IL-15R alpha knockout mice are hyperglycemic and insulin-resistant. PMID: 26269523
Signaling through IL-15Ralpha regulates the development of gammadelta-17 cells early in ontogeny. PMID: 26195801
lower frequencies of IL-15Ralpha expression in PBMCs of Behcet's disease-like symptomatic mice PMID: 23618691
Epidermal IL-15Ralpha acts as an endogenous antagonist of psoriasiform inflammation in mouse and man. PMID: 24019554
a greater mitochondrial density and mitochondrial DNA content was found in fast muscles from IL-15-Receptor-alpha KO mice that was not due to the presence of smaller muscle fibers. PMID: 23116661
IL-15 is produced and secreted only as a heterodimer with IL-15Ralpha. PMID: 22496150
Stage 3 thymic invariant natural killer NKT cells are specifically reduced in IL-15Ralpha-deficient mice, whereas the acquisition of natural killer (NK) receptors occurs at stage 3 IL15-dependent thymic cell development. PMID: 21709149
Different levels of IL-15 trans-presentation are required for different natural killer (NK) cell developmental events to reach full maturation status PMID: 21715685
IL-15 receptor alpha has a role in endurance, fatigability, and metabolic characteristics of mouse fast skeletal muscles PMID: 21765213
The disrupted circadian rhythm of temperature and activity in the IL15Ralpha KO mice after LPS suggests that upregulated IL15 receptors may serve a beneficial role to counteract the consequences of neuroinflammation. PMID: 20981579
IL15 is essential to maintain neurochemical homeostasis and thereby plays a role in preventing neuropsychiatric symptoms. PMID: 20724079
IL15Ralpha is co-localized with co-receptor IL2Rgamma and IL-15 in neuroinflamed cerebral endothelia. PMID: 21155807
IL15Ralpha is essential for normal anxiety-like behavior, but inhibition of gliosis in the fearless IL15Ralpha knockout mice or IL15 treatment of normal mice did not acutely modulate behavioral performance as tested PMID: 20600810
T cells expressing low-avidity T cell receptors respond poorly to IL-15/IL-15Ralpha complex, which correlates with a poor homeostatic proliferative response to lymphopenia. PMID: 21041729
LFA-1 signal defect-induced CD8(+) T cell apoptosis is associated with reduced CD27 costimulation and IL-15R survival signal, elucidating the molecular mechanism associated with LFA-1 signaling in effector and memory CD8(+) T cell survival PMID: 20569988
Identified four novel IL15Ralpha splice variants in mouse cerebral microvessels composing the BBB, and showed their differential distribution and functions in endothelial signaling in response to IL15. PMID: 20374432
Data suggest that activation of hypothalamic neurons by IL-15 in mice contributes to thermoregulation and modifies the metabolic phenotype. PMID: 20012227
IL-15Ralpha(-/-) renal cells exhibited a higher rate of cisplatin-induced apoptosis PMID: 19958157
IL15R signaling is essential to maintain hippocampal GABA concentrations, at least partially involving reduced GABA turnover, and results in facilitation of memory consolidation. PMID: 20357123
Expression of soluble IL-15Ralpha (sIL-15Ralpha) mRNA declined 5-fold with age PMID: 19854259
Neuronal development in olfactory epithelium (OE) is inhibited by interleukin (IL)-15Ralpha deficiency because of decreased proliferative activity but not promoted apoptosis of OE cells PMID: 20021484
Data show that the short lifespan of T(H)17 cells was associated with small amounts of the antiapoptotic protein Bcl-2, the IL-15 receptor and the receptor CD27. PMID: 19935657
IL-15R alpha deficiency results in specific decreases in both the number and Bcl-2 content of CD8+ T cells. PMID: 11777964
Treatment with soluble interleukin-15Ralpha exacerbates intracellular parasitic infection by blocking the development of memory CD8+ T cell response. (IL-15R alpha) PMID: 12045244
Expression of the high-affinity receptor, IL-15R alpha, on T cells is dispensable for the generation or maintenance of memory CD8(+) T cells. By contrast, IL-15R alpha expression on cells other than T cells is absolutely critical for this function. PMID: 12671073
These findings demonstrate that natural killer (NK) cell-independent interleukin-15 receptor alpha (IL-15Ralpha) expression is critical for maintaining peripheral NK cells, while IL-15Ralpha expression on NK cells is not required for this function. PMID: 12695489
SOCS1 functions as an indispensable attenuator of IL-15 receptor signaling in developing CD8+ thymocytes PMID: 12907450
IL-15 receptor alpha chain expression on developing natural killer (NK) cells is not critically important for the development of CD94/NKG2+ lytic NK cells, but is required for further acquisition of Ly-49 receptors by NK cells. PMID: 14607906
IL-15 receptor alpha expression by murine dendritic cells is critical for natural killer (NK) cell activation and presentation of IL-15 in trans to NK cells during NK cell priming. PMID: 15356102
IL-15Ralpha defines homeostatic niches for NK and memory CD8+ T cells by controlling both the production and the presentation of IL-15 in trans to NK and CD8+ memory T cells PMID: 15452177
IL-15Ralpha can act in cis in addition to acting in trans to present IL-15 to responding cells. PMID: 18796634
IL-15 expression alone in TC-1 tumor cells partially inhibits tumor growth whereas IL-15 and IL-15Ralpha expression together completely inhibits tumor growth in a natural killer 1.1-positive cell- and CD8-positive T cell-dependent manner. PMID: 19050240
The decidual leucocytes also up-regulated IL-15 and IL-15Ralpha in stromal fibroblasts which could produce a niche for uNK cells allowing proliferation within and recruitment into the uterus. PMID: 19088135
Dendritic cell derived-exosomes promoted an IL-15Ralpha- and NKG2D-dependent NK cell proliferation and activation respectively, resulting in anti-metastatic effects PMID: 19319200
Results indicate that potential nuclear function of cleaved interleukin-2 receptor beta-chain and IL-15 receptor alpha-chain subunits is not plausible. PMID: 19329337
E4BP4 acted in a cell-intrinsic manner 'downstream' of the interleukin 15 receptor (IL-15R) and through the transcription factor Id2 PMID: 19749763
IL-15R alpha expression on macrophages but not dendritic cells (DCs) supports the early transition of antigen specific effector CD8(+) T cells to memory cells PMID: 19913445

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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