Recombinant Mouse IL12 (IL12A & IL12B Heterodimer) Protein
Beta LifeScience
SKU/CAT #: BLPSN-2654
Recombinant Mouse IL12 (IL12A & IL12B Heterodimer) Protein
Beta LifeScience
SKU/CAT #: BLPSN-2654
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | |
Host Species | Mouse |
Accession | P43431 |
Synonym | Il-12a, Il-12b, IL-12p35, Il-12p40, Il12p40, Ll12a, p35, p40 |
Description | A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a His tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. |
Source | HEK293 |
Predicted N Terminal | Arg 23 & Met 23 |
AA Sequence | Met 1-Ala 215 |
Molecular Weight | The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12B is Fc chimera, comprises 758 (204 + 554) a.a. and has a calculated molecular mass of 85.9 (23.1 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 75 kDa respectively in SDS-PAGE under reducing conditions. |
Purity | >85% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured by its ability to mouse IL12RB2-His in a functional ELISA.2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.2-1 ng/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |