Recombinant Mouse HVEM Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2477
Recombinant Mouse HVEM Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2477
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Product Overview
Tag | His&Fc |
Host Species | Mouse |
Accession | NP_849262.1 |
Synonym | HVEM Tumor Necrosis Factor Receptor Superfamily Member 14; Herpes Virus Entry Mediator A; Herpesvirus Entry Mediator A; HveA; Tumor Necrosis Factor Receptor-Like 2; TR2; CD270; TNFRSF20; HVEA; HVEM |
Background | Herpesvirus entry mediator (HVEM) is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity. HVEM is highly expressed on naive CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils. It functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha. Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation and contributes to Th1 inflammation and cardiac allograft rejection. In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation and dampens intestinal inflammation. HVEM enhances the development of CD8+ T cell memory and Treg function. It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD166 promotes epitheilal integrity and host defense. The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding. |
Description | A DNA sequence encoding the extracellular domain (Met 1-Gln 206) of mouse HVEM (NP_849262.1) precursor was fused with C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
Source | CHO Stable Cells |
Predicted N Terminal | Pro 40 |
AA Sequence | Met 1-Gln 206 |
Molecular Weight | The recombinant mouse HVEM /Fc is a disulfide-linked homodimeric Protein after removal of the signal peptide. The reduced monomer consists of 415 amino acids and predicts a molecular mass of 46.4 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rmHVEM/Fc monomer is approximately 65 KDa due to the glycosylation. |
Purity | Greater than 85% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its binding ability in a functional ELISA.Immobilized mouse HVEM-Fc can bind biotinylated mouse BTLA-Fc,The EC50 of biotinylated mouse BTLA-Fc is 152-228 ng/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C |
Usage | For Research Use Only |
Storage | Store recombinant protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network. Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation. Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions. Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response. In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and proinflammatory cytokines. Upon binding to CD160 on activated CD4+ T cells, downregulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response. May interact in cis (on the same cell) or in trans (on other cells) with BTLA. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells. |
Subcellular Location | Cell membrane; Single-pass type I membrane protein. |
Tissue Specificity | Expressed at mucosal sites including colon and pulmonary epithelial cells. Expressed in naive T cells. |