USA  Tel: 1-201-888-7983
Email: info@betalifesci.com

Recombinant Mouse CD14 Protein

Cat No.: BL-0009PS
CD14 Mouse Recombinant, Sf9
Description Related products
tag His
host species Mouse
synonym Monocyte differentiation antigen CD14, Myeloidcell-specific leucine-rich glycoprotein, CD14.
background CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.
description CD14 expressed in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 357a.a. (16-366a.a.) and having a molecular weight of 38.3kDa.(Molecular size on SDS-PAGE will appear at approximately 40-57kDa).CD14 is expressed with a 6a.a. His tag at C-Terminus and purified by unique purification methods.
source Sf9
AA sequence SPAPPEPCEL DEESCSCNFS DPKPDWSSAF NCLGAADVEL YGGGRSLEYLLKRVDTEADL GQFTDIIKSL SLKRLTVRAA RIPSRILFGA LRVLGISGLQ ELTLENLEVT GTAPPPLLEATGPDLNILNL RNVSWATRDA WLAELQQWLK PGLKVLSIAQ AHSLNFSCEQ VRVFPALSTLDLSDNPELGE RGLISALCPLKFPTLQVLAL RNAGMETPSG VCSALAAARV QLQGLDLSHN SLRDAAGAPS CDWPSQLNSL NLSFTGLKQV PKGLPAKLSVLDLSYNRLDR NPSPDELPQV GNLSLKGNPF LDSESHSEKF NSGVVTAGAP SSQAVALSGT LALLLGDRLF VHHHHHH.
purity >90% as determined by SDS-PAGE.
endotoxin <1.0 EU per μg by the LAL method.
formulation CD14 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
stability Recombinant protein is stable for 12 months at -70℃
usage For Research Use Only
storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Online Inquiry
For quote, please fill in the online form or send email to info@betalifesci.com.
$50 Coupon