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Recombinant Mouse Cathepsin S Protein (His Tag)

Cat No.: BLPSN-0608
Cat S Protein, Mouse; Cats Protein, Mouse
Description Related products
tag His
host species Mouse
accession AAB94925.1
synonym Cat S, Cats
background Cathepsin S (CTSS), one of the lysosomal proteinases, has many important physiological functions in the nervous system, especially in process of extracellular matrix degradation and endocellular antigen presentation. CTSS is synthesized as inactive precursor of 331 amino acids consisting of a 15-aa signal peptide, a propeptide of 99 aa, and a mature polypeptide of 217 aa. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. Cathepsin S is expressed in the lysosome of antigen presenting cells, primarily dendritic cells, B-cells and macrophages. Compared with other lysosomal cysteine proteases, cathepsin S has displayed some unique characteristics. Cathepsin S is most well known for its critical function in the proteolytic digestion of the invariant chain chaperone molecules, thus controlling antigen presentation to CD4+ T-cells by major histocompatibility complex (MHC) class II molecules or to NK1.1+ T-cells via CD1 molecules. Cathepsin S also appears to participate in direct processing of exogenous antigens for presentation by MHC class II to CD4+ T-cells, or in cross-presentation by MHC class I molecules to CD8+ T-cells. In addition, although direct evidence is still lacking, in its secreted form cathepsin S is implicated in degradation of the extracellular matrix, which may contribute to the pathology of a number of diseases, including arthritis, atherosclerosis, neurological diseases and chronic obstructive pulmonary disease.
description A DNA sequence encoding the full length of mouse CTSS (AAB94925.1) (Met 1-Ile 340) was expressed, with a C-terminal His tag.
source HEK293
predicted n terminal Val 18
AA sequence Met 1-Ile 340
molecular weight The recombinant mouse CTSS consists of 334 a.a. and predicts a molecular mass of 37.6 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCTSS is approximately 33-38 KDa.
purity >90% as determined by SDS-PAGE
endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
bioactivity Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVENval-WRK (Dnp)-NH2, AnaSpec, Catalog # 27114.The specific activity is >300 pmoles/min/ug.(Activation description: The enzyme achieves its activity under acidic pH)
formulation Lyophilized from sterile PBS, pH 7.4.
stability The recombinant proteins are stable for up to 1 year from date of receipt at -70℃.
usage For Research Use Only
storage Store the protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Cat No. Product Name Source Detail Inquiry
BLPSN-0609 Recombinant Rat Cathepsin S Protein (His Tag) HEK293
BLPSN-0607 Recombinant Human Cathepsin S Protein (His Tag) HEK293
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