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Recombinant Mouse Cathepsin B Protein (His Tag)

Cat No.: BLPSN-0597
CB Protein, Mouse
Description Related products
tag His
host species Mouse
accession P10605
synonym CB
background Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.
description A DNA sequence encoding the mouse CTSB (P10605) (Met 1-Phe 339) was fused with a His tag at the C-terminus.
source HEK293
predicted n terminal His 18
AA sequence Met 1-Phe 339
molecular weight The secreted recombinant mouse CTSB (pro form) consists of 333 a.a. and has a predicted molecular mass of 36.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CTSB is approximately 35-43 kDa due to glycosylation.
purity >95% as determined by SDS-PAGE
endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
bioactivity Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008). The specific activity is >2,000 pmoles/min/ug.
formulation Lyophilized from sterile PBS, pH 7.4.
stability The recombinant proteins are stable for up to 1 year from date of receipt at -70℃.
usage For Research Use Only
storage Store the protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Cat No. Product Name Source Detail Inquiry
BLPSN-0598 Recombinant Rat Cathepsin B Protein (His Tag) HEK293
BLPSN-0596 Recombinant Human Cathepsin B Protein (His Tag) HEK293
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