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Recombinant Mouse ACE2 Protein (His Tag)

Cat No.: BLPSN-0043
2010305L05Rik Protein, Mouse
Description Related products
tag His
host species Mouse
accession Q8R0I0
synonym 2010305L05Rik
background Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.
description A DNA sequence encoding the mouse ACE2 isoform 1 (Q8R0I0-1) extracellular domain (Met 1-Thr 740) was fused with a His tag at the C-terminus.
source HEK293
predicted n terminal Gln 18
AA sequence Met 1-Thr 740
molecular weight The secreted recombinant mouse ACE2 comprises 734 a.a. and has a predicted molecular mass of 85 kDa. As a result of glycosylation, it migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
purity >95% as determined by SDS-PAGE
endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
bioactivity Please contact us for detailed information
formulation Lyophilized from sterile 25mM Tris, 150mM NaCl, 1mM ZnCl2, pH 7.5.
stability The recombinant proteins are stable for up to 1 year from date of receipt at -70℃.
usage For Research Use Only
storage Store the protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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