Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 (aa 1-725, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-5445
Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 (aa 1-725, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-5445
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | MERS-CoV |
Description | The spike protein S1 (Human betacoronavirus 2c EMC/2012)( AFS88936.1)(Met1-Glu725) was fused with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Tyr 18 |
AA Sequence | Met1-Glu725 |
Molecular Weight | The recombinant spike protein S1 (Human betacoronavirus 2c EMC/2012) comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions. |
Purity | Greater than 95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725)(Cat: BLPSN-5445) at 10 μg/ml (100 μl/well) can bind biotinylated human CD26 (Cat: BLPSN-0833)._x000D_ The EC50 of of biotinylated DPP4 (Cat: BLPSN-0833) is 0.6-1.39 μg/ml. 2.Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) (Cat: BLPSN-5445) at 10 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: BLPSN-0834). The EC50 of biotinylated Fc-DPP4 is 0.02-0.05 μg/ml. |
Formulation | This product was lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol. |
Stability | Recombinant antigens are stable for up to 1 year from date of receipt at -80°C |
Applications | ELISA; immunogen; WB, etc. |
Usage | For Research Use Only |
Storage | Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Please avoid repeated freeze-thaw cycles. |