Recombinant IpaD Protein (Tagged)
Beta LifeScience
SKU/CAT #: BLA-9799P
Recombinant IpaD Protein (Tagged)
Beta LifeScience
SKU/CAT #: BLA-9799P
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Host Species | Shigella flexneri |
Accession | P18013 |
Synonym | Invasin ipaD secreted by the Mxi-Spa machinery, required for entry of bacteria into epithelial cells |
Description | Recombinant IpaD Protein (Tagged) was expressed in E.coli. It is a Full length protein |
Source | E.coli |
AA Sequence | MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSLTMLNDT LHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILS RNEYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYL KVYEHAVSSYTQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALE ELKEKYKDKPLYPANNTVSQEQANKWLTELGGTIGKVSQKNGGYVVSINM TPIDNMLKSLDNLGGNGEVVLDNAKYQAWNAGFSAEDETMKNNLQTLVQK YSNANSIFDNLVKVLSSTISSCTDTDKLFLHF |
Molecular Weight | 57 kDa including tags |
Purity | >90% SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation | Liquid Solution |
Stability | The recombinant protein samples are stable for up to 12 months at -80°C |
Reconstitution | See related COA |
Unit Definition | For Research Use Only |
Storage Buffer | Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Target Details
Target Function | Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via TTSS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm. Along with IpaB, is essential for both blocking secretion through the Mxi/Spa translocon in the absence of a secretion-inducing signal, and for controlling the level of secretion in the presence of this signal. |
Subcellular Location | Secreted. Note=Secreted via the type III secretion system (TTSS). Localizes to the tip of the external secretion needle that is part of the TTSS apparatus. |
Protein Families | Invasin protein D family |
Database References | KEGG: sfl:CP0126 |
Gene Functions References
- IpaD, the putative needle-tip protein of the S. flexneri type III secretion system, has been crystallized; in-drop proteolysis resulted in the production of several new crystal forms. PMID: 16946465
- IpaD forms part of the structure at the needle tip in Shigella flexner and antibodiesblock entry of bacteria into epithelial cells. PMID: 17110044