Recombinant Human WT1 Protein (Flag Tag)
Beta LifeScience
SKU/CAT #: BL-120PC
Recombinant Human WT1 Protein (Flag Tag)
Beta LifeScience
SKU/CAT #: BL-120PC
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Flag |
Host Species | Human |
Accession | NM_000378. |
Background | WT-1, the product of Wilms' tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, inclusion or exclusion of exon 5 and the three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of WT1 protein (1-3). Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms' tumor. WT1 (-KTS) appears to have different binding affinity to both DNA and RNA comparing to the +KTS form (4, 5). |
Description | Recombinant Human WT1 Protein was expressed in Baculovirus-insect cell. This protein is fused with a Flag tag and purified using our unique purification methods. |
Source | Baculovirus-insect cell |
Molecular Weight | 55.7 kDa. |
Purity | >90% purity by SDS-PAGE |
Endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Recombinant Human WT1 Protein is supplied in 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA |
Usage | For Research Use Only |
Storage | Stored the protein at -70°C before use. Avoid repeated freeze thaw cycles. |