Recombinant Human WT1 Protein (Flag Tag)
Beta LifeScience
SKU/CAT #: BL-119PC
Recombinant Human WT1 Protein (Flag Tag)
Beta LifeScience
SKU/CAT #: BL-119PC
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Flag |
Host Species | Human |
Accession | NM_024426.4. |
Background | WT-1, the product of Wilms' tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein (1-3). Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms' tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive (4). This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator (5, 6). |
Description | Recombinant Human WT1 Protein was expressed in Baculovirus-insect cell. This protein is fused with a Flag tag and purified using our unique purification methods. |
Source | Baculovirus-insect cell |
Molecular Weight | 54.6 kDa. |
Purity | >90% purity by SDS-PAGE |
Endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Recombinant Human WT1 Protein is supplied in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. |
Usage | For Research Use Only |
Storage | Stored the protein at -70°C before use. Avoid repeated freeze thaw cycles. |