Tel: 1-201-888-7983
E-mail: info@betalifesci.com
| tag | N/A |
| host species | Human |
| accession | P01375 |
| synonym | TNFα / TNFA Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2 |
| background | TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease. |
| description | Recombinant Human Tumor Necrosis Factor alpha / TNFa is expressed by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed. |
| source | E.coli |
| AA sequence | Val77-Leu233 |
| purity | >99% as determined by HPLC. |
| endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| bioactivity | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/ml. |
| formulation | Recombinant Human TNFα / TNFA Protein was lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.0. |
| reconstitution | Dissolve the lyophilized TNFA protein in distilled water. |
| usage | For Research Use Only |
| storage | Human TNFα / TNFA protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
