Recombinant Human SV40 T-Antigen Protein (His Tag)
Beta LifeScience
SKU/CAT #: BL-117PC
Recombinant Human SV40 T-Antigen Protein (His Tag)
Beta LifeScience
SKU/CAT #: BL-117PC
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Simian Virus |
Accession | AAG39214.1. |
Background | The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth (1-4). A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain (5,6). The p53-interacting domain is also essential for binding p300/CBP (7). Phosphorylation of TAg by several kinases may regulate its function both positively and negatively (8). |
Description | Recombinant Human SV40 T-Antigen Protein was expressed in Baculovirus-insect cell. This protein is fused with a His tag and purified using our unique purification methods. |
Source | Baculovirus-insect cell |
AA Sequence | MDKVLNREES LQLMDLLGLE RSAWGNIPLM RKAYLKKCKE FHPDKGGDEE KMKKMNTLYK KMEDGVKYAH QPDFGGFWDA TEIPTYGTDE WEQWWNAFNE ENLFCSEEMP SSDDEATADS QHSTPPKKKR KVEDPKDFPS ELLSFLSHAV FSNRTLACFA IYTTKEKAAL LYKKIMEKYS VTFISRHNSY NHNILFFLTP HRHRVSAINN YAQKLCTFSF LICKGVNKEY LMYSALTRDP FSVIEESLPG GLKEHDFNPE EAEETKQVSW KLVTEYAMET KCDDVLLLLG MYLEFQYSFE MCLKCIKKEQ PSHYKYHEKH YANAAIFADS KNQKTICQQA VDTVLAKKRV DSLQLTREQM LTNRFNDLLD RMDIMFGSTG SADIEEWMAG VAWLHCLLPK MDSVVYDFLK CMVYNIPKKR YWLFKGPIDS GKTTLAAALL ELCGGKALNV NLPLDRLNFE LGVAIDQFLV VFEDVKGTGG ESRDLPSGQG INNLDNLRDY LDGSVKVNLE KKHLNKRTQI FPPGIVTMNE YSVPKTLQAR FVKQIDFRPK DYLKHCLERS EFLLEKRIIQ SGIALLLMLI WYRPVAEFAQ SIQSRIVEWK ERLDKEFSLS VYQKMKFNVA MGIGVLDWLR NSDDDDEDSQ ENADKNEDGG EKNMEDSGHE TGIDSQSQGS FQAPQSSQSV HDHNQPYHIC RGFTCFKKPP TPPPEPET |
Molecular Weight | 83.3 kDa. |
Purity | >95% purity by SDS-PAGE |
Endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Recombinant Human SV40 T-Antigen Protein is supplied in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. |
Usage | For Research Use Only |
Storage | Stored the protein at -70°C before use. Avoid repeated freeze thaw cycles. |