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Recombinant Human Siglec-15 / SIGLEC15 Protein (His Tag)

Cat No.: BL-2151NP
Description Related products
tag His
host species Human
accession Q6ZMC9
synonym Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3; CD33L3
background Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.
description Recombinant Human Siglec-15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a 6His tag at the C-terminus.
source Human Cells
AA sequence Phe20-Thr263
molecular weight 27.4kDa. AP Mol Mass 30-40kDa, reducing conditions.
purity >95% as determined by reducing SDS-PAGE.
endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
bioactivity Immobilized Human Siglec-15-His(Cat#BL-2151NP) at 10μg/ml (100 μl/well) can bind Biotinylated Mouse MAG-His (Cat#BL-1176NP). The ED50 of Human Siglec-15-His(Cat#BL-2151NP) is 6-20 ug/ml
formulation Human SIGLEC15 protein was lyophilized from a 0.2 μm filtered solution of PBS, 150mM NaCl, 5% Trehalose, 0.3% CHAPS, pH 7.4.
stability Recombinant Human Siglec-15 protein is stable for 1 year at -70℃
reconstitution Dissolve the lyophilized SIGLEC15 protein in distilled water.
usage For Research Use Only
storage Lyophilized SIGLEC15 protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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