tag |
His |
host species |
Human |
accession |
Q6ZMC9 |
synonym |
Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3; CD33L3 |
background |
Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts. |
description |
Recombinant Human Siglec-15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a 6His tag at the C-terminus. |
source |
Human Cells |
AA sequence |
Phe20-Thr263 |
molecular weight |
27.4kDa. AP Mol Mass 30-40kDa, reducing conditions. |
purity |
>95% as determined by reducing SDS-PAGE. |
endotoxin |
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
bioactivity |
Immobilized Human Siglec-15-His(Cat#BL-2151NP) at 10μg/ml (100 μl/well) can bind Biotinylated Mouse MAG-His (Cat#BL-1176NP). The ED50 of Human Siglec-15-His(Cat#BL-2151NP) is 6-20 ug/ml |
formulation |
Human SIGLEC15 protein was lyophilized from a 0.2 μm filtered solution of PBS, 150mM NaCl, 5% Trehalose, 0.3% CHAPS, pH 7.4. |
stability |
Recombinant Human Siglec-15 protein is stable for 1 year at -70℃ |
reconstitution |
Dissolve the lyophilized SIGLEC15 protein in distilled water. |
usage |
For Research Use Only |
storage |
Lyophilized SIGLEC15 protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |