Recombinant Human S100A3 Protein (His & MBP Tag)

Beta LifeScience SKU/CAT #: BLPSN-4120

Recombinant Human S100A3 Protein (His & MBP Tag)

Beta LifeScience SKU/CAT #: BLPSN-4120
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Product Overview

Tag His&MBP
Host Species Human
Accession P33764
Synonym S100E
Background Protein S1-A3, also known as Protein S-1E, S1 calcium-binding protein A3, S1A3 and S1E, is a member of the S-1 family. S1A3 / S1E contains 2EF-hand domains. S1A3 / S1E is highly expressed in the differentiating cuticular cells within the hair follicle and organized into mature hair cuticles. High concentrations of S1A3 homotetramer might provide the millimolar level of Ca2+ required for hair cuticular barrier formation. S1A3 / S1E is a unique member of the Ca2+-binding S1 protein family with the highest cysteine content and affinity for Zn2+. S1A3 / S1E binds both calcium and zinc. S1A3 / S1E probably binds 2 zinc ions per molecule. It may be involved in calcium-dependent cuticle cell differentiation and hair shaft formation. S1A3 plays an important role in calcium-dependent processes leading to hair shaft formation. S1A3 / S1E is a unique protein among all members of the calcium-binding S1 family, is specifically expressed at the inner endocuticle of human hair fibers. Upon hair damage, S1A3 / S1E is released from hair fibers and possibly destabilizes the hair tissue architecture.
Description A DNA sequence encoding the human S100A3 (P33764) (Met 1-Gln 101) was fused with an N-terminal His-tagged MBP tag at the N-terminus.
Source E.coli
Predicted N Terminal Met
AA Sequence Met 1-Gln 101
Molecular Weight The recombinant human S100A3/MBP fusion protein consists of 498 a.a. and has a calculated molecular mass of 55.3 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin Please contact us for more information.
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, 20% glycerol, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Binds both calcium and zinc. May be involved in calcium-dependent cuticle cell differentiation, hair shaft and hair cuticular barrier formation.
Subcellular Location Cytoplasm.
Protein Families S-100 family
Database References
Tissue Specificity Skin specific, specifically expressed at the inner endocuticle of hair fibers.

Gene Functions References

  1. S100A3 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  2. One disulfide bridge is between Cys30 in the N-terminal pseudo-EF-hand and Cys68 in the C-terminal EF-hand (SS1), and another disulfide bridge attaches Cys99 in the C-terminal coil structure to Cys81 in helix IV (SS2). PMID: 21377473
  3. crystal structure of S100A3 at 1.7-A resolution PMID: 12045193
  4. structure was solved by MIRAS phasing PMID: 12136135
  5. Purification and characterization of the S100A3 protein from human hair cuticles. PMID: 12470658
  6. cytoplasmic S100A3 within the cuticular layer is mostly co-localized with the type III isoform of peptidylarginine deiminase (PAD3) PMID: 18083705

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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