Recombinant Human PLA2G2D Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3840
Recombinant Human PLA2G2D Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3840
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Fc |
Host Species | Human |
Accession | Q9UNK4 |
Synonym | PLA2G2D, PLA2IID, SPLASH, sPLA2-IID, sPLA2S, phospholipase A2 group IID |
Background | PLA2G2D (Phospholipase A2 Group IID) is a Protein Coding gene. Diseases associated with PLA2G2D include Superior Mesenteric Artery Syndrome and Duodenal Obstruction. Among its related pathways are Oxytocin signaling pathway and Activation of cAMP-Dependent PKA. Gene Ontology (GO) annotations related to this gene include calcium ion binding and phospholipase A2 activity. An important paralog of this gene is PLA2G2A. |
Description | A DNA sequence encoding the human PLA2G2D (Met1-Cys145) was expressed with the Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Gly 21 |
AA Sequence | Met1-Cys145 |
Molecular Weight | The recombinant human PLA2G2D/Fc is a disulfide-linked homodimer. The reduced monomer comprises 366 amino acids and has a predicted molecular mass of 41.5 kDa. The apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions. |
Purity | >85% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Recombinant Human PLA2G2D Protein (Fc Tag) was lyophilized from sterile PBS, pH 7.4. |
Stability | Recombinant Human PLA2G2D protein is stable up to 1 year at -70°C |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Secretory calcium-dependent phospholipase A2 that primarily targets extracellular lipids, exerting anti-inflammatory and immunosuppressive functions. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. In draining lymph nodes, selectively hydrolyzes diacyl and alkenyl forms of phosphatidylethanolamines, releasing omega-3 polyunsaturated fatty acids (PUFAs) such as eicosapentaenoate and docosahexaenoate that are precursors of the anti-inflammatory lipid mediators, resolvins. During the resolution phase of acute inflammation drives docosahexaenoate-derived resolvin D1 synthesis, which suppresses dendritic cell activation and T-helper 1 immune response. May act in an autocrine and paracrine manner. Via a mechanism independent of its catalytic activity, promotes differentiation of regulatory T cells (Tregs) and participates in the maintenance of immune tolerance. May contribute to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. |
Subcellular Location | Secreted. |
Protein Families | Phospholipase A2 family |
Database References | |
Tissue Specificity | Highly expressed in pancreas and spleen and less abundantly in colon, thymus, placenta, small intestine, and prostate. |
Gene Functions References
- found to induce PLA2G2D expression in mice and in human monocyte-derived macrophages PMID: 26392224
- sPLA(2)-IID is present at the head and midpiece in the human sperm, and its activation seems to be involved in acrosomal exocytosis. PMID: 22240557
- phospholipid bilayer permeabilization by the hsPLA2GIID is independent of catalytic activity. PMID: 22490726
- The Gly80Ser polymorphism may be associated with the body weight loss seen in chronic obstructive pulmonary disease patients. PLA2G2D-Ser enhances the expression of IL-6 and IL-8 compared with PLA2G2D-Gly. PMID: 19365107
- TNF-alpha & IFN-gamma induce gene expression of a novel secretory PLA(2)IIDin human airway epithelial cells. The possibility that it is involved in cytokine-mediated inflammation in the respiratory tract is inferred. PMID: 12396716
- The GIID sPLA2 is clustered on human chromosome 1 and is differentially expressed in tissues, suggesting it has unique function. PMID: 15052324
- overexpression of human secretory phospholipase A2 group IIA leads to an enhanced delivery of cholesterol from phospholipolysed lipoproteins to the liver PMID: 15379211
- These results suggest that sPLA2-IID may be one of the susceptibility genes that contribute to body weight loss in patients with COPD. PMID: 16002569
- lipopolysaccharides inhibit interferon gamma-induced gene expression of secretory phospholipase A2 type IID in human monocyte-derived macrophages PMID: 16897354
- The distribution pattern of sPLA2S in intact spermatozoa might be an additional parameter for evaluating sperm quality PMID: 18958346