Recombinant Human NKG2D Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3519
Recombinant Human NKG2D Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3519
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Fc |
Host Species | Human |
Accession | NP_031386.2 |
Synonym | CD314, D12S2489E, KLR, NKG2-D, NKG2D |
Background | NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP1 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis. |
Description | A DNA sequence encoding the human NKG2D (NP_031386.2) (Phe78-Val216) was expressed with the Fc region of human IgG1 at the N-terminus. |
Source | CHO Stable Cells |
Predicted N Terminal | Met |
AA Sequence | Phe78-Val216 |
Molecular Weight | The recombinant human NKG2D/Fc comprises 418 a.a. and has a predicted molecular mass of 46.5 kDa. The apparent molecular mass of the protein is approximately 50-64 and 37 kDa in SDS-PAGE under reducing conditions. |
Purity | (81.4+13.9)% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Immobilized human ULBP1-His at 10 ug/ml (100 ul/well) can bind human S4-Fc3L3-NKG2D, The EC50 of human S4-Fc3L3-NKG2D is 0.39-0.91 ug/ml. |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |