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| tag | Fc |
| host species | Human |
| accession | P24394 |
| synonym | IL4 Rα Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; IL-4-binding protein; IL4-BP; IL4R; IL4RA |
| background | Interleukin 4 Receptor alpha (IL4-Ra) is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. Mature human IL4-Ra consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif. IL4-Ra plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. Soluble forms of IL4-Ra, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL-4 bioactivity. IL4-Ra is a component of two distinct receptor complexes and shows species selectivity between human and mouse. It can associate with the common gamma chain (γc) to form the IL-4 responsive type I receptor in which γc increases the affinity for IL-4 and enables signaling. It can alternatively associate with IL13-Ra1 to form the type II receptor which is responsive to both IL-4 and IL-13. The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize γc. |
| description | Recombinant Human Interleukin-4 Receptor Subunit Alpha is expressed by our Mammalian expression system and the target gene encoding Met26-Gln231 is expressed with a Fc tag at the C-terminus. |
| source | Human Cells |
| AA sequence | Met26-Gln231 |
| purity | >95% as determined by reducing SDS-PAGE. |
| endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| bioactivity | Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is less than 20 ng/ml. |
| formulation | Recombinant Human IL4 Rα Protein was lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| reconstitution | Dissolve the lyophilized protein in distilled water. |
| usage | For Research Use Only |
| storage | Human IL4 Rα protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Cat No. | Product Name | Source | Detail | Inquiry |
| BL-1846NP | Recombinant Human IL4 Rα Protein (mFc Tag) | Human Cells |  |
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