tag |
Fc |
host species |
Human |
accession |
P24394 |
synonym |
IL4 Rα Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; IL-4-binding protein; IL4-BP; IL4R; IL4RA |
background |
Interleukin 4 Receptor alpha (IL4-Ra) is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. Mature human IL4-Ra consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif. IL4-Ra plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. Soluble forms of IL4-Ra, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL-4 bioactivity. IL4-Ra is a component of two distinct receptor complexes and shows species selectivity between human and mouse. It can associate with the common gamma chain (γc) to form the IL-4 responsive type I receptor in which γc increases the affinity for IL-4 and enables signaling. It can alternatively associate with IL13-Ra1 to form the type II receptor which is responsive to both IL-4 and IL-13. The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize γc. |
description |
Recombinant Human Interleukin-4 Receptor Subunit Alpha is expressed by our Mammalian expression system and the target gene encoding Met26-Gln231 is expressed with a Fc tag at the C-terminus. |
source |
Human Cells |
AA sequence |
Met26-Gln231 |
purity |
>95% as determined by reducing SDS-PAGE. |
endotoxin |
<0.1 ng/µg (1 EU/µg) as determined by LAL test. |
bioactivity |
Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is less than 20 ng/ml. |
formulation |
Recombinant Human IL4 Rα Protein was lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
reconstitution |
Dissolve the lyophilized protein in distilled water. |
usage |
For Research Use Only |
storage |
Human IL4 Rα protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |