Recombinant Human IL35 (IL12A & EBI3 Heterodimer) Protein
Beta LifeScience
SKU/CAT #: BLPSN-2881
Recombinant Human IL35 (IL12A & EBI3 Heterodimer) Protein
Beta LifeScience
SKU/CAT #: BLPSN-2881
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | |
Host Species | Human |
Accession | NP_005746.2 & NP_000873.2 |
Synonym | CLMF, IL-12A, NFSK, NKSF1, P35 |
Background | IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity. |
Description | A DNA sequence encoding the human IL27B (NP_005746.2)(Met1-Lys229) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human IL12A (NP_000873.2)(Met1-Ser219) was fused with a His tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human IL27B&IL12A heterodimer was purified. |
Source | HEK293 |
Predicted N Terminal | Arg 21 & Arg 23 |
AA Sequence | Met1-Lys229 |
Molecular Weight | The recombinant heterodimer of human IL27B&IL12A comprises 425 (217+208) a.a. and has a calculated molecular mass of 48.3(24.3+24) KDa. |
Purity | >85% as determined by SDS-PAGE. |
Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |