Recombinant Human IL18 Protein (pro form, GST Tag), inactive

Collections: Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Tag	inactive
Host Species	Human
Accession	Q14116
Synonym	IGIF, IL-18, IL-1g, IL1F4, Interleukin 18
Background	Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-alpha and IL-1beta, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description	A DNA sequence encoding the pro form of human IL18 (Q14116) (Met1-Asp193) was fused with the GST tag at the N-terminus.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Met1-Asp193
Molecular Weight	The recombinant pro form of human IL18 consists of 419 a.a. and has a predicted molecular mass of 48.6 kDa.
Purity	>85% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.5.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (Probable). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (Probable).
Subcellular Location	Cytoplasm. Secreted.
Protein Families	IL-1 family
Database References	HGNC: 5986 OMIM: 600953 KEGG: hsa:3606 STRING: 9606.ENSP00000280357 UniGene: Hs.83077

Gene Functions References

The results of the linkage disequilibrium test showed that the -137G/C and -607G/T sites had no linkage disequilibrium in the present study. PMID: 29926984
SNPs associated with genetically determined high activity of TLR5 among patients with CD and genetically determined high IL-12 and IL-18 levels among patients with UC. PMID: 28139755
The adipose tissue IL-18R/IL-18 expression is enhanced in obesity which associates with proinflammatory gene signature and insulin resistance in these individuals. PMID: 28508444
Our study implied that elevated IL-18 expression and downregulation of GAS5 in serums might contribute to insulin resistance in polycystic ovary syndrome patients. PMID: 29648472
Studied serum level of interleukin 18 (IL18) in HIV and Hepatitis C (HCV) coinfection and monoinfection. Found shown that IL-18 is increased in HIV/HCV coinfection when compared with monoinfection and that this increase is likely due to additive sensing of both viruses. PMID: 27927859
not associated with obesity in this study PMID: 28634744
multiple myeloma patients with IL-18 (rs16944) TT had the highest hemoglobin concentration PMID: 30211233
These results suggest that increased IL-18 secretion from larger numbers of neutrophils elicits mechanical hyperalgesia. PMID: 29353540
The results of this study suggests that IL-18 rs187238 and rs1946518 SNPs may be the cause of the AA susceptibility. PMID: 29349811
Studied association of single nucleotide polymorphisms of interleukin-18 (IL 18) and lower extremity deep venous thrombosis in a Chinese population. PMID: 29105174
PI3K is a signal linker between L-selectin and PSGL-1 in IL-18 transcriptional activation at the promoter level. PMID: 29218606
IL-18 is a susceptibility gene for the disease in southern Chinese children. PMID: 30059753
Postoperative serum Cystatin C, IL-18 and Uric acid after 24h were significantly associated with acute kidney injury PMID: 29627485
IL-18 is elevated in polycystic ovary syndrome patients, even in lean ones, and is correlated with insulin resistance and atherosclerotic cardiovascular risk PMID: 29105530
HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18. PMID: 28231690
The IL18 promoter SNP, rs1946518, is a potential risk factor of periodontitis among young Japanese women. PMID: 29129846
The results of this study suggest that IL18 rs187238 and rs1946518 polymorphisms may be associated with an increased risk of gestational diabetes mellitus as well as with BMI in pregnant women. PMID: 28580570
This is the first study to demonstrate that higher pre-treatment IL-18 is associated with HBeAg seroconversion in patients with HIV/HBV co-infection after 48 weeks of HBV cART, which suggests a role for the inflammasome in a favorable response to treatment. PMID: 28195558
Polymorphisms in the IL-18 gene are associated with viral load in Hepatitis B virus . PMID: 30045250
higher level of IL-18 was observed in the monocyte supernatant of the extremely preterm group during late-onset sepsis PMID: 29042061
Increased production of IL-18 in the gut-associated lymphoid tissue and peripheral blood of HIV infected patients. PMID: 29672590
this review discusses IL-18 biological properties and role in disease pathogenesis PMID: 29247988
IL-18 mRNA expression was increased significantly in chronic myeloid leukemia patients PMID: 29097263
these results suggest that the HIV-induced IL-18 plays a role in increased intestinal permeability and microbial translocation observed in HIV-infected individuals. PMID: 29601578
IL-18 gene variants may serve as important biomarkers in predicting the occurrence of nasopharyngeal carcinoma in Asian population. PMID: 29185028
The association of polymorphisms -137 (rs187238) and -607 (rs1946518) in the interleukin-18 (IL-18) promoter with risk of hepatocellular carcinoma (HCC). PMID: 28000712
These findings indicate that the IL-18 rs1946518 SNP in the donor liver is a risk factor for developing bacterial infection after liver transplantation. PMID: 29061223
Serum levels of IL-18 in nephropathic patient significantly rise and Serum levels of IL-18 might be a predictor factor of progression of diabetic nephropathy. PMID: 27663212
A higher IL-18 immunostaining in stroma of IBC and LABC was observed in comparison with tumor cells, but stromal immunoexpression was similar between IBC and LABC. PMID: 28766916
IL-18 was increased in lung myeloid cells in severe chronic obstructive pulmonary disease. PMID: 28830544
a large proportion blood basophils from patients with asthma express IL-18 and IL-18BP. mast cells and basophils are implicated in the pathogenesis of asthma via an IL-18-related mechanism. PMID: 29505668
On PICU day 1, interleukin-18 predicted acute kidney injury with area under the curve=0.82, but neutrophil gelatinase-associated lipocalin and liver fatty acid binding protein predicted acute kidney injury with area under the curve of less than or equal to 0.69; on PICU day 2, area under the curve was higher. Interleukin-18 and liver fatty acid binding protein on day 1 predicted prolonged acute kidney injury. PMID: 28430754
Data show that tumor-derived IL-18 induced PD-1 expression on NK cells and is associated with poor prognosis in triple-negative breast cancer. PMID: 28415798
IL-23, alone and in combination with IL-18 and sRAGE, identified bacterial meningitis with excellent accuracy. Following validation, these markers could aid clinicians in diagnosis of bacterial meningitis and decision-making regarding prolongation of antibiotic therapy PMID: 29394248
cardiac IL-18 and circulating IL-18 are involved in the pathogenesis of Fabry cardiomyopathy and left ventricular hypertrophy. PMID: 27888626
Erdr1 is negatively expressed relative to Il-18 in psoriatic lesional skin. PMID: 26990875
rs187238 was not associated with T1D in the Euro-Brazilian population. PMID: 28973736
IL-18 (mRNA) in skeletal muscle appears to be involved in the regulation of intramuscular lipid metabolism and hypertriglyceridemia PMID: 29342149
The evidence for influenza A virus activation via an indirect, IL-18-dependent mechanism indicates that MAIT cells are protective in influenza, and also possibly in any human disease process in which inflammation and IL-18 production occur. PMID: 27543331
The development of urticaria, asthma, dermatitis, rhinitis, and eosinophilic disorders all have demonstrated correlations to increased IL-18 levels either in the tissue or systemically. IL-18 represents a novel site of immune regulation in not only allergic conditions, but also autoimmune diseases and other instances of aberrant immune functioning. PMID: 27496752
Results suggest that a common functional IL18 haplotype associated with heightened proinflammatory responses confers susceptibility to stress-related depression and anxiety through effects on threat-related amygdala function, a risk pathway specific to women. PMID: 27430614
human platelets contain transcripts for the IL-18 gene. They synthesize the cytokine de novo, process and release it upon activation. PMID: 27914933
abnormal IL-18 expression is induced by genital infection and induces damage to male reproductive capacity, thereby causing male infertility PMID: 26856767
IL-18 level was found to be significantly elevated in CAD patients compared with control individuals PMID: 28059484
The serum levels of IL-37, which were correlated with antibody production and the serum levels of total IL-18 and IL-18BP, were elevated in the patients with primary Sjogren's syndrome. PMID: 28057714
an imbalance in the production of IL-18 and its antagonist (an increase in the production of IL-18 with a decrease, no increase or an insufficient increase in the production of IL-18BP) has been described in many chronic inflammatory diseases in humans. PMID: 26898120
among systemic sclerosis patients in China, dyspnea was significantly associated with IL-18 -607C/A genotype frequency PMID: 27098064
We conclude that IL-18 protein and messenger RNA levels are increased in the endometrium of patients with PCOS, and this effect is correlated with body mass index. PMID: 27313119
Serum IL-18 levels are significantly elevated in depressive patients. PMID: 27455146
IL-18 cooperates with IL-15 to promote group 3 innate lymphoid cell (ILC3) proliferation and IL-22 production; describe an IL-18-induced, NF-kappaB-mediated mechanism that regulates IL-22 in ILC3s; at steady-state, IL-18 produced by dendritic cells mediates IL-22 production by ILC3s to help maintain normal tissue integrity PMID: 28842466

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Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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