tag |
Fc |
host species |
Human |
accession |
NP_001973.2 |
synonym |
ERBB3, ErbB-3, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, erb-b2 receptor tyrosine kinase 3, FERLK |
background |
Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene.ErbB3 is a member of the epidermal growth factor receptor (EGFR/ERBB) family of receptor tyrosine kinases. The kinase-impaired ErbB3 is known to form active heterodimers with other members of the ErbB family, most notably the ligand binding-impaired ErbB2.ERBB3 (Erb-B2 Receptor Tyrosine Kinase 3) is a Protein Coding gene. Diseases associated with ERBB3 include Lethal Congenital Contracture Syndrome 2 and Erythroleukemia, Familial. Among its related pathways are PI3K/AKT activation and RET signaling. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is ERBB4. |
description |
A DNA sequence encoding the extracellular domain (Met 1-Thr 643) of human ErbB3 (NP_001973.2) precursor was expressed with the C-terminal fused Fc region of human IgG1. |
source |
HEK293 |
predicted n terminal |
Ser 20 |
AA sequence |
Met 1-Thr 643 |
molecular weight |
The mature recombinant human ErbB3/Fc chimera is a disulfide-linked homodimeric protein after the removal of signal peptide. The monomer consists of 862 amino acids and has a calculated molecular mass of 95.4 kDa. As a result of glycosylation, the recombinant monomer migrates as an approximately 130-140 kDa protein in SDS-PAGE under reducing conditions. |
purity |
>90% as determined by SDS-PAGE |
endotoxin |
< 1.0 EU per μg of the protein as determined by the LAL method |
bioactivity |
Measure by its ability to bind with human NRG1-β1 in a functional ELISA. |
formulation |
Recombinant Human HER3 / ERBB3 Protein (Fc Tag) was lyophilized from sterile PBS, pH 7.4 |
stability |
Recombinant Human HER3 / ERBB3 protein is stable up to 1 year at -70℃ |
usage |
For Research Use Only |
storage |
Store the protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |