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| tag | His |
| host species | Human |
| accession | P01588 |
| synonym | EPO Erythropoietin; Epoetin; EPO |
| background | Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. Erythropoietin is a member of the EPO/TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. |
| description | Recombinant Human Erythropoietin is expressed by our Mammalian expression system and the target gene encoding Ala28-Arg193 is expressed with a His tag at the C-terminus. |
| source | Human Cells |
| AA sequence | Ala28-Arg193 |
| purity | >95% as determined by reducing SDS-PAGE. |
| endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| bioactivity | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is less than 5 ng/ml |
| formulation | Recombinant Human EPO Protein was lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| reconstitution | Dissolve the lyophilized EPO protein in distilled water. |
| usage | For Research Use Only |
| storage | Human EPO protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
