tag |
His |
host species |
Human |
accession |
P01040 |
synonym |
CSTA Cystatin-A; Cystatin-AS; Stefin-A; CSTA; STF1; STFA |
background |
Human Cystatin A (CSTA) is a member of family 1 of the cystatin superfamily, which is characterized by lacking of disulphide bonds an carbohydrates. Cystatin A is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as Cathepsins B, H and L. Cystatin A is also implicated in a number of disease states. Due to altered proteolytic state in cancer progression, Cystatin A may play a role in the proteolytic pathways. |
description |
Recombinant Human Cystatin A is expressed by our E.coli expression system and the target gene encoding Ile2-Phe98 is expressed with a His tag at the N-terminus. |
source |
E.coli |
AA sequence |
Ile2-Phe98 |
purity |
>95% as determined by reducing SDS-PAGE. |
endotoxin |
<0.1 ng/µg (1 EU/µg) as determined by LAL test. |
formulation |
Recombinant Human CSTA Protein was lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, pH 8.0. |
reconstitution |
Dissolve the lyophilized protein in distilled water. |
usage |
For Research Use Only |
storage |
Human CSTA protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |