Recombinant Human coronavirus spike glycoprotein Protein (isolate HKU1) (aa 1-760, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4875
Recombinant Human coronavirus spike glycoprotein Protein (isolate HKU1) (aa 1-760, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4875
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Product Overview
Tag | His |
Host Species | Human coronavirus spike glycoprotein Protein |
Accession | Q5MQD0 |
Description | The N-terminal fragment of human coronavirus (isolate HKU1) spike glycoprotein (YP_173238.1) (Met 1-Arg 760), corresponding to the S1 region, was fused with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Gly 16 |
AA Sequence | Met 1-Arg 760 |
Molecular Weight | The secreted recombinant human coronavirus spike glycoprotein (aa 1-760) comprises 756 amino acids with a predicted molecular mass of 85.8 kDa. As a result of high glycosylation, the apparent molecular mass of the recombinant protein is approximately 130-140 kDa in SDS-PAGE under reducing conditions. |
Purity | Greater than 90% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation | This product was lyophilized from sterile PBS, pH 7.4 |
Stability | Recombinant antigens are stable for up to 1 year from date of receipt at -80°C |
Applications | ELISA; immunogen; WB, etc. |
Usage | For Research Use Only |
Storage | Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Please avoid repeated freeze-thaw cycles. |
Target Details
Target Function | attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis. |
Subcellular Location | Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. |
Protein Families | Betacoronaviruses spike protein family |
Database References | KEGG: vg:3200426 |
Gene Functions References
- the crystal structure of HKU1 spike glycoprotein C-terminal domain at 1.9 A resolution, is reported.[Spike]. PMID: 28534504
- 4.0 A resolution structure of the trimeric HKU1 S protein determined using single-particle cryo-electron microscopy PMID: 26935699