tag |
Fc |
host species |
Human |
accession |
Q08722 |
synonym |
CD47 Leukocyte Surface Antigen CD47; Antigenic Surface Determinant Protein OA3; Integrin-Associated Protein; IAP; Protein MER6; CD47; MER6 |
background |
CD47(Integrin-Associated Protein,IAP) is a 40 ‑ 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes. |
description |
Recombinant Human Leukocyte Surface Antigen CD47 is expressed by our Mammalian expression system and the target gene encoding Gln19-Pro139 is expressed with a Fc tag at the C-terminus. |
source |
Human Cells |
AA sequence |
Gln19-Pro139 |
purity |
>95% as determined by reducing SDS-PAGE. |
endotoxin |
<0.1 ng/µg (1 EU/µg) as determined by LAL test. |
bioactivity |
Measured by its ability to bind Human SIRPA in functional ELISA. The ED50 for this effect is less than 200 ng/ml. |
formulation |
Recombinant Human CD47 Protein was lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
reconstitution |
Dissolve the lyophilized protein in distilled water. |
usage |
For Research Use Only |
storage |
Human CD47 protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |