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| tag | Fc |
| host species | Human |
| accession | Q08722 |
| synonym | CD47 Leukocyte Surface Antigen CD47; Antigenic Surface Determinant Protein OA3; Integrin-Associated Protein; IAP; Protein MER6; CD47; MER6 |
| background | CD47(Integrin-Associated Protein,IAP) is a 40 ‑ 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes. |
| description | Recombinant Human Leukocyte Surface Antigen CD47 is expressed by our Mammalian expression system and the target gene encoding Gln19-Pro139 is expressed with a Fc tag at the C-terminus. |
| source | Human Cells |
| AA sequence | Gln19-Pro139 |
| purity | >95% as determined by reducing SDS-PAGE. |
| endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| bioactivity | Measured by its ability to bind Human SIRPA in functional ELISA. The ED50 for this effect is less than 200 ng/ml. |
| formulation | Recombinant Human CD47 Protein was lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| reconstitution | Dissolve the lyophilized protein in distilled water. |
| usage | For Research Use Only |
| storage | Human CD47 protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
