Recombinant Human CD27 Protein
Beta LifeScience
SKU/CAT #: BL-0032PS
Recombinant Human CD27 Protein
Beta LifeScience
SKU/CAT #: BL-0032PS
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Synonym | Tumor Necrosis Factor Receptor Superfamily Member 7, T-Cell Activation Antigen CD27, CD27 Molecule, CD27 Antigen, T Cell Activation Antigen S152, CD27L Receptor, TNFRSF7, S152, Tp55, T14. |
Background | CD27 belongs to the TNF-receptor superfamily. CD27 is necessary for initiation and long-term maintenance of T cell immunity. CD27 binds to ligand CD70, and has a crucial role in regulating B-cell activation and immunoglobulin synthesis. The CD27 receptor transduces signals which result in the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 mediate the signaling process of CD27. CD27-binding protein (SIVA), which is a proapoptotic protein, can bind to the CD27 receptor and is believed to have a significant role in the apoptosis induced by CD27. |
Description | CD27 Human Recombinant expressed in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 413a.a. (21-191a.a.) and having a molecular weight of 46.4kDa (Molecular size on SDS-PAGE will appear at approximately 28-57kDa).CD27 is fused with a 239a.a. hIgG-His tag at C-Terminus and purified by unique purification methods. |
Source | Sf9 |
AA Sequence | ADPTPAPKSC PERHYWAQGK LCCQMCEPGT FLVKDCDQHR KAAQCDPCIP GVSFSPDHHT RPHCESCRHC NSGLLVRNCT ITANAECACR NGWQCRDKEC TECDPLPNPS LTARSSQALS PHPQPTHLPY VSEMLEARTA GHMQTLADFR QLPARTLSTH WPPQRSLCSS DFIRLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH. |
Purity | >85.0% as determined by SDS-PAGE. |
Endotoxin | <1.0 EU per μg by the LAL method. |
Formulation | CD27 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH7.4) and 10% glycerol. |
Stability | Recombinant protein is stable for 12 months at -70°C |
Usage | For Research Use Only |
Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |