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Recombinant Human CD27 Protein (Fc Tag)

Cat No.: BLPSN-0838
S152 Protein, Human; S152. LPFS2 Protein, Human; S152.LPFS2 Protein, Human; T14 Protein, Human; TNFRSF7 Protein, Human; Tp55 Protein, Human
Description Related products
tag Fc
host species Human
accession P26842
synonym S152, S152. LPFS2, S152.LPFS2, T14, TNFRSF7, Tp55
background CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD7 on lymphocytes and dendritic cells. The CD27-CD7 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.Immune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsCo-stimulatory Immune Checkpoint Targets ImmunotherapyCancer ImmunotherapyTargeted Therapy
description A DNA sequence encoding the human CD27 (P26842) (Met1-Ile192) was expressed, fused with the Fc region of rabbit IgG at the C-terminus.
source HEK293
predicted n terminal Ala 20
AA sequence Met1-Ile192
molecular weight The recombinant human CD27/rFc is a disulfide-linked homodimer. The reduced monomer comprises 396 a.a. and has a predicted molecular mass of 44.4 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions.
purity >95% as determined by SDS-PAGE
endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
bioactivity Measured by its binding ability in a functional ELISA.Immobilized human CD27-his at 10 ug/mL (100 ul/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 95-150 ng/mL.
formulation Lyophilized from sterile PBS, pH 7.4.
stability The recombinant proteins are stable for up to 1 year from date of receipt at -70℃.
usage For Research Use Only
storage Store the protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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