tag |
His |
host species |
Human |
synonym |
T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, Erythrocyte receptor, Rosette receptor, CD2 antigen, CD2, T11, SRBC. |
background |
CD2 which is also known as E-rosette receptor, T11 and lymphocytefunction antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated after CD2 stimulation. CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis, apoptosis in activated peripheral T cells, and regulation of T cell anergy. |
description |
CD2 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 209a.a. (25-209 a.a) and having a molecular weight of 23.8kDa.CD2 is fused to a 24a.a. His-tag at N-terminus and purified by unique purification methods. |
source |
E.coli |
AA sequence |
MGSSHHHHHH SSGLVPRGSH MGSMKEITNA LETWGALGQD INLDIPSFQM SDDIDDIKWE KTSDKKKIAQ FRKEKETFKE KDTYKLFKNG TLKIKHLKTD DQDIYKVSIY DTKGKNVLEK IFDLKIQERV SKPKISWTCI NTTLTCEVMN GTDPELNLYQ DGKHLKLSQR VITHKWTTSLSAKFKCTAGN KVSKESSVEP VSCPEKGLD. |
purity |
>85.0% as determined by SDS-PAGE. |
endotoxin |
<1.0 EU per μg by the LAL method. |
formulation |
CD2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea. |
stability |
Recombinant protein is stable for 12 months at -70℃ |
usage |
For Research Use Only |
storage |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |