tag |
His |
host species |
Human |
synonym |
Antigen-presenting glycoprotein CD1d, R3G1, CD antigen: CD1d, Differentiation Antigen CD1-Alpha-3, T-Cell Surface Glycoprotein CD1d, Thymocyte Antigen CD1D, CD1A, R3, CD1d Molecule, CD1D Antigen, D Polypeptide, CD1d Antigen, R3G1, HMC Class I, Antigen-Like Glycoprotein CD1D, Antigen-Presenting Glycoprotein CD1d. |
background |
CD1D, also known as antigen-presenting glycoprotein CD1d, is a transmembrane glycoprotein which belongs to the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1d-presented lipid antigens activate a special class of T cells, familiar as natural killer T (NKT) cells, during the interaction with the T-cell receptor present on NKT membranes. Once activated, NKT cells rapidly produce Th1 &Th2 cytokines, usually represented by interleukin 4 production. |
description |
CD1D expressed in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 290a.a. (20-301 a.a.) and having a molecular weight of 32.9kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).CD1Dis expressed with an 8a.a. His tag at C-Terminus and purified by unique purification methods. |
source |
Sf9 |
AA sequence |
EVPQRLFPLR CLQISSFANS SWTRTDGLAW LGELQTHSWS NDSDTVRSLK PWSQGTFSDQ QWETLQHIFR VYRSSFTRDV KEFAKMLRLS YPLELQVSAG CEVHPGNASN NFFHVAFQGK DILSFQGTSW EPTQEAPLWV NLAIQVLNQD KWTRETVQWL LNGTCPQFVS GLLESGKSELKKQVKPKAWL SRGPSPGPGR LLLVCHVSGF YPKPVWVKWM RGEQEQQGTQ PGDILPNADE TWYLRATLDV VAGEAAGLSC RVKHSSLEGQ DIVLYWGGSY TSLEHHHHHH. |
purity |
>85.0% as determined by SDS-PAGE. |
endotoxin |
<1.0 EU per μg by the LAL method. |
formulation |
CD1D protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
stability |
Recombinant protein is stable for 12 months at -70℃ |
usage |
For Research Use Only |
storage |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |