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| tag | Fc |
| host species | Human |
| accession | P15391 |
| synonym | CD19 B-Lymphocyte Antigen CD19; B-Lymphocyte Surface Antigen B4; Differentiation Antigen CD19; T-Cell Surface Antigen Leu-12; CD19 |
| background | CD19 is a single-pass type I membrane protein containing 2 Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. CD19 primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. CD19 Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) which is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. |
| description | Recombinant Human CD19 is expressed by our Mammalian expression system and the target gene encoding Pro20-Lys291 is expressed with a Fc tag at the C-terminus. |
| source | Human Cells |
| AA sequence | Pro20-Lys291 |
| purity | >80% as determined by reducing SDS-PAGE. |
| endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| bioactivity | Measured by its ability to binding CD19 used funtional ELISA. The ED50 for this effect is less than 20 ug/ml |
| formulation | Recombinant Human CD19 Protein was lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1%BSA, 0.05%NaN3, pH 7.4. |
| reconstitution | Dissolve the lyophilized protein in distilled water. |
| usage | For Research Use Only |
| storage | Human CD19 protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
