Recombinant Human BCL10 Protein (His Tag)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Synonym	c-E10; CARMEN; CIPER; CLAP; mE10 and B-cell CLL/lymphoma 10.
Background	Bcl-10 is an intracellular signaling protein identified from the t(1;14)(p22;q32) breakpoint in MALT lymphomas. The human Bcl10 gene encodes a protein of 233 amino acids containing an N-terminal caspase recruitment domain (CARD) that mediates self-oligomerization and the C-terminal region of Bcl-10 (1-3). Bcl 10 is expressed ubiquitously with high expression levels in lymphoid tissues and in the developing central nerve system (4-6). Mutations have been found in cases of follicular lymphoma and diffuse large B cell lymphoma (7). Bcl-10 is a positive regulator of antigen-receptor induced NF-kB activation and Bcl-10-initiated activation of NF-kB can be inhibited by cotransfection of dominant-negative mutants of TRAF2, NIK, IKKa or IkBa (3-5, 8).
Description	Recombinant Human BCL10 Protein was expressed in Baculovirus-insect cell. This protein is fused with a His tag and purified using our unique purification methods.
Source	Baculovirus-insect cell
AA Sequence	MEPTAPSLTE EDLTEVKKDA LENLRVYLCE KIIAERHFDH LRAKKILSRE DTEEISCRTSSRKRAGKLLD YLQENPKGLD TLVESIRREK TQNFLIQKIT DEVLKLRNIK LEHLKGLKCSSCEPFPDGAT NNLSRSNSDE SNFSEKLRAS TVMYHPEGES STTPFFSTNS SLNLPVLEVGRTENTIFSST TLPRPGDPGA PPLPPDLQLE EEGTCANSSE MFLPLRSRTV SRQ
Purity	>90% purity by SDS-PAGE
Endotoxin	<0.1 ng/µg (1 EU/µg) as determined by LAL test.
Bioactivity	1 unit equals 1 nanogram of purified protein.
Formulation	The human BCL10 protein is supplied in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
Usage	For Research Use Only
Storage	Stored the protein at -70°C before use. Avoid repeated freeze thaw cycles.

Target Details

Target Function	Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation. Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Recruited by activated CARD domain-containing proteins: homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex. This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Activated by CARD9 downstream of C-type lectin receptors; CARD9-mediated signals are essential for antifungal immunity. Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK.
Subcellular Location	Cytoplasm, perinuclear region. Membrane raft.
Database References	HGNC: 989 OMIM: 137245 KEGG: hsa:8915 STRING: 9606.ENSP00000271015 UniGene: Hs.193516
Associated Diseases	Immunodeficiency 37 (IMD37); Lymphoma, mucosa-associated lymphoid type (MALTOMA)
Tissue Specificity	Ubiquitous.

Gene Functions References

Results show that GSK3beta modulates serine phosphorylation of BCL10. PMID: 29358699
BCL10 forms CARMA1-BCL10-MALT1-TRAF6 signalosome and BCL10 polymerizes in a unidirectional manner. PMID: 29382759
The results suggest that the involvement of BCL10 in DNA damage-induced NF-kappaB is through the recruitment of TRAF6. PMID: 28717989
results define molecular determinants that control the production of Lin(Ub)n-Bcl10, an important signaling intermediate in TCR and oncogenic CARD11 signaling. PMID: 27777308
Psoriasis mutations disrupt CARD14 autoinhibition promoting BCL10-MALT1-dependent NF-kappaB activation PMID: 27071417
BCL10 promoted DNA double-strand breaks repair, enhancing cell survival after DNA damage. PMID: 26771713
Data show that caspase recruitment domain-containing protein 11/B-cell CLL/lymphoma 10/mucosa-associated lymphoid tissue lymphoma translocation gene 1 signaling drives lymphoproliferation through NF-kappa B and c-Jun N-terminal kinase activation. PMID: 26668357
BCL10 is not essential for actin polymerization after fibroblast FcgammaR stimulation. PMID: 26774590
characterization of zebrafish (Danio rerio) Bcl10 PMID: 25849213
we identified BCL10 as a bona fide target of BCR-induced linear ubiquitylation and demonstrated an important role of the linear ubiquitin ligase HOIP in BCR-induced phosphorylation PMID: 26038114
The results of this study indicate that inherited BCL10 deficiency should be considered in patients with combined immunodeficiency with B cell, T cell, and fibroblast defects. PMID: 25365219
B-cell lymphoma/leukemia 10 promotes oral cancer progression through STAT1/ATF4/S100P signaling pathway. PMID: 24681956
BCL10 induces cleavage of MALT1 at R149 in 293T cells. PMID: 25105596
Overexpression of CARMA-BCL10-MALT in T-ALL may contribute to the constitutive cleavage and inactivation of A20, which enhances NF-kappaB signaling and may be related to T-ALL pathogenesis. PMID: 25384343
BCL10 delivers UBC13 to RNF8/RNF168 to regulate ubiquitination-mediated double-strand break signaling and repair. PMID: 24732096
elevated in cholesteatoma PMID: 24702227
A novel susceptibility locus on 1p22 was discovered, which implicates BCL10 as a new susceptibility gene for leprosy. PMID: 23784377
Combining crystallography, nuclear magnetic resonance, and electron microscopy, we reveal the structure of the Bcl10 CARD filament and the mode of interaction between CARMA1 and Bcl10 PMID: 24074955
BCL10 expression is a useful marker for acinar cell differentiation, particularly in the diagnosis of endoscopic ultrasound-guided fine-needle aspiration specimens of Acinar cell carcinomas of the pancreas PMID: 23530562
Bcl10 links saturated fat overnutrition with hepatocellular NF-kB activation and insulin resistance. PMID: 22708078
BCL10 was commonly down-regulated in peripheral T cell lymphomas, suggest the T-cell receptor signaling cascade for future characterization. PMID: 22818167
identify Bcl10 as an early coordinator of NF-kappaB-mediated immune response with endosomal trafficking and signaling to F-actin remodeling PMID: 23153494
Results indicate that NF-kappaB binding to the BCL10 promoter can lead to prolonged activation of the carrageenan-induced inflammatory cascade by a transcriptional mechanism involving an NF-kappaB-BCL10 loop. PMID: 22579587
A proapoptotic role for protein kinase C zeta in the binding and phosphorylating Bcl10 at the nuclear envelope. PMID: 22812606
BCL10 plays an important role in controlling the growth of cervical cancer cells through NF-kappaB dependent cyclin D1 regulation. PMID: 22564715
FOXO3a promotes cell survival via BCL10/NF-kappaB in serum starvation PMID: 22474286
identify MIB2 as a novel component of the activated BCL10 signaling complex and a missing link in the BCL10-dependent NF-kappaB signaling pathway. PMID: 21896478
findings indicate that BCL10 phosphorylations act upstream of phosphorylations of NIK, TAK1, and IkappaBalpha and differentially affect the canonical and noncanonical pathways of NF-kappaB activation PMID: 21700900
Findings demonstrate that CaN functions as a critical signaling molecule during Th cell activation, regulating Bcl-10 phosphorylation and NF-kappaB activation. PMID: 21674474
study shows CaMKII is recruited to the immunological synapse where it interacts with and phosphorylates Bcl10; propose a mechanism whereby Ca(2+) signals can be integrated at the immunological synapse through CaMKII-dependent phosphorylation of Bcl10 PMID: 21513986
Data show that CARMA3 and Bcl10 contributed to several characteristics of EGFR-associated malignancy, including proliferation, survival, migration, and invasion. PMID: 21406399
Lipopolysaccharide induces activation of both canonical and non-canonical pathways of NF-kappaB and the non-canonical pathway requires phosphorylations of BCL10 (serine 138) and NIK. PMID: 20466000
A novel mutation of Bc1-10 gene in ocular adnexal MALT lymphoma was detected in Chinese patients. PMID: 18307945
BCL10 nuclear expression is common in ocular adnexal mucosa-associated lymphoid tissue lymphomas. PMID: 19035248
activation of NF-kappaB by CXCR4 occurs through Carma3/Bcl10/Malt1 (CBM) complex in OSCC. loss of components of CBM complex in HNSCC can inhibit SDF-1 alpha induced phosphorylation and degradation of IkappaBalpha. PMID: 20695076
Interaction of calmodulin with Bcl10 modulates NF-kappaB activation. PMID: 20439115
A specific single nucleotide polymorphism in the BCL10 gene may be responsible for the tumorigenesis of intracranial germinomas in Japanese individuals. PMID: 19690445
Results suggest that the NFkappaB regulator BCL10 is an IL-2-independent STAT5 target gene. PMID: 19709433
These findings indicate an upstream signaling role for BCL10, in addition to its effects on IKKgamma, the regulatory component of the IKK signalosome, and a requirement for BCL10 in both canonical and noncanonical pathways of NF-kappaB activation. PMID: 19897484
vimplicated in apoptosis, and it has been suggested that mutated forms gain oncogenic activity. The occurrence of genomic BCL10 mutations in gastric MALT-type lymphomas was investigated. PMID: 11830492
Mutations, relatively common in lymphomas, are extremely rare in malignant cartilaginous tumors. PMID: 11836626
REVIEW: Genetic alterations involving BCL10 underlying the pathogenesis of MALT lymphoma PMID: 11960389
There is a lack of BCL10 mRNA mutation in lymphold malignancies. PMID: 12017308
association of mutations with aberrant BCL10 localization in the nucleus in nasal NK/T-cell lymphomas PMID: 14523480
Bcl10 is post-translationally modified by Rip2 and has a role in T-cell signaling PMID: 14638696
Nuclear expression of BCL10 is unlikely to correlate with the API2-MALT1 fusion gene in ocular adnexal MALT lymphoma. PMID: 14674990
Together, these findings suggest that Bcl10 nuclear expression may modulate gene expression and Bcl10 is a potential transcriptional activator apart from its traditional roles that have been found. PMID: 15207693
BinCard inhibits BCL10-mediated activation of NF-kappa B. PMID: 15637807
The ability of Bcl10 expression to prevent B-cell antigen receptor-induced growth arrest and apoptosis of WEHI-231 cells was dependent on NF-kappaB activation. PMID: 15878976
nucleocytoplasmic shuttling of MALT1 and BCL10 complex may indicate that these molecules are involved not only in the nuclear factor kappaB (NF-kappaB) pathway but also in other biologic functions in lymphocytes PMID: 16123224

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