Recombinant dog IL8 Protein

Beta LifeScience SKU/CAT #: BLA-0880P

Recombinant dog IL8 Protein

Beta LifeScience SKU/CAT #: BLA-0880P
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Product Overview

Host Species Dog
Accession P41324
Synonym (Ala-IL-8)77 (Ser-IL-8)72 9E3 Beta thromboglobulin like protein C-X-C motif chemokine 8 CEF-4 chemokine, CXC motif, ligand 8 CXCL8 Emoctakin GCP-1 GCP/IL-8 protein I GCP/IL-8 protein II GCP/IL-8 protein III GCP/IL-8 protein IV GCP/IL-8 protein V GCP/IL-8 protein VI GCP1 Granulocyte chemotactic protein 1 IL-8 IL-8(1-77) IL-8(9-77) IL8 IL8/NAP1 form I IL8/NAP1 form II IL8/NAP1 form III IL8/NAP1 form IV IL8/NAP1 form V IL8/NAP1 form VI IL8_HUMAN Inteleukin 8 LECT LUCT Lymphocyte-derived neutrophil-activating factor LYNAP MDNCF MDNCF-b MDNCF-c MONAP Monocyte derived neutrophil activating peptide Monocyte derived neutrophil chemotactic factor Monocyte-derived neutrophil chemotactic factor Monocyte-derived neutrophil-activating peptide NAF NAP 1 NAP-1 NAP1 Neutrophil activating peptide 1 Neutrophil activating protein 1 Neutrophil-activating factor Neutrophil-activating protein 1 Protein 3 10C Protein 3-10C SCYB 8 SCYB8 Small inducible cytokine subfamily B member 8 T cell chemotactic factor T-cell chemotactic factor TSG 1 TSG1
Description Recombinant dog IL8 Protein was expressed in E.coli. It is a Full length protein
Source E.coli
AA Sequence AVLSRVSSELRCQCIKTHSTPFHPKYIKELRVIDSGPHCENSEIIVKLFN GNEVCLDPKEKWVQKVVQIFLKKAEKQDP
Molecular Weight 9 kDa
Purity >95% purity as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity The biological activity was determined by a chemotaxis bioassay using Human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.15-0.75 ng/ml.
Formulation Lyophilised
Stability The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution See related COA
Unit Definition For Research Use Only
Storage Buffer Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.

Target Details

Target Function IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Database References

Gene Functions References

  1. Analysis of ROC suggests the use of serum levels of MCP-1 and IL-7 as predictors of the occurrence of complications with an AUC of 0.906 and 0.896 respectively and linear combinations of MCP-1, KC-Like, IL-7 and GM-CSF with values up to AUC = 0.983 PMID: 29304171
  2. These observations indicate that the individual activation of ERK2 and JNK1 pathways contributes to TNF-alpha-induced IL-8 expression in synovial fibroblasts. PMID: 28806729
  3. data suggest that CCL2 acts as a PMNs chemotactic factor as well as CXCL8, and both CCL2 and CXCL8 facilitate the infiltration of PMNs into the joints of idiopathic polyarthritis PMID: 27863550
  4. The study findings suggest that both CCL2 and CXCL8 are involved in the pathogenesis of canine idiopathic pulmonary fibrosis. PMID: 26231926
  5. High CXCL8 blood concentrations might be related to the breed predisposition of the West Highland white terrier for canine idiopathic pulmonary fibrosis. PMID: 26267090
  6. IL-8-positive macrophages were significantly increased in large polyps compared to controls PMID: 24148828
  7. hemangiosarcoma derived IL-8 may play a role in tumor development by modulating the tumor microenvironment PMID: 24582862
  8. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
  9. serum and tumor levels of IL-8 and IL-10 in dogs bearing benign and malignant mammary tumors, including dogs with inflammatory mammary cancer, for a better understanding of this disease PMID: 23351639
  10. Sixty-one percent (28/46) of the samples from canine stifle osteoarthritis had IL-8 mRNA expression in contrast to 4% (1/24) in the control stifle joints PMID: 16102844
  11. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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