Recombinant Cynomolgus CTLA-4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1472
Recombinant Cynomolgus CTLA-4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1472
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Fc |
Host Species | Cynomolgus |
Accession | Q9BDC4 |
Synonym | GSE, CD152, IDDM12, CELIAC3, CTLA-4. |
Background | CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. |
Description | A DNA sequence encoding the cynomolgus/rhesus CTLA4 (Q9BDC4) (Met1-Asp161) was produced with the Fc region of human IgG1 at the C-terminus. Cynomolgus and Rhesus CTLA4 sequences are identical. |
Source | HEK293 |
Predicted N Terminal | Ala 37 |
AA Sequence | Met1-Asp161 |
Molecular Weight | The recombinant cynomolgus/rhesus CTLA4 is a disulfide-linked homodimer. The reduced monomer comprises 366 amino acids and has a calculated molecular mass of 40.4 KDa.The apparent molecular mass of the protein is approximately 52 KDa respectively in SDS-PAGE. |
Purity | Greater than 95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human B7-1-His at 10 ug/ml (100 ul/well) can bind Cynomolgus CTLA4-Fc, The EC50 of Cynomolgus CTLA4-Fc is 6.0-14.5 ng/ml. |
Formulation | Lyophilized from sterile PBS, pH 7.4 |
Stability | Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C |
Usage | For Research Use Only |
Storage | Store recombinant protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |