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| tag | His |
| host species | 2019-nCoV |
| accession | QHD43416.1 |
| synonym | 2019-nCov RBD Protein; 2019-nCoV Spike RBD Protein |
| background | Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| description | Recombinant 2019-nCoV S Protein RBD-SD1 is produced by our Mammalian expression system and the target gene encoding Arg319-Ser591 is expressed with a 6His tag at the C-terminus. |
| source | Human Cells |
| AA sequence | Arg319-Ser591 |
| molecular weight | 31.4kDa. AP Mol Mass: 35-43kDa, reducing conditions |
| purity | >95% as determined by reducing SDS-PAGE. |
| bioactivity | Immobilized Human ACE-2-Fc(Cat#BLS-0997NP) at 10μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-SD1-His(Cat#BLPSN-10013P).The ED50 of Recombinant 2019-nCoV S Protein RBD-SD1-His(Cat#BLPSN-10013P) is 0.64 ug/ml. |
| formulation | Recombinant 2019-nCoV S Protein RBD-SD1 was supplied as a 0.2 μM filtered solution of PBS,PH7.4. |
| applications | Immunogen, calibrator or standard. |
| purification | Affinity purification chromatography. |
| Sensitivity | For Research Use Only |
| Detection Limit | Store at < -20°C, stable for 12 months after receipt. Please Avoid repeated freeze-thaw cycles. |
