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| tag | His |
| host species | 2019-nCoV |
| accession | QHD43416.1 |
| synonym | S protein RBD; 2019-nCoV S protein RBD |
| background | 2019 Novel Coronavirus (2019-nCoV) is a virus (more specifically, a coronavirus) identified as the cause of an outbreak of respiratory illness first detected in Wuhan, China. |
| description | A DNA sequence encoding the 2019-nCoV Spike Protein (RBD) was expressed in HEK293 Cells and fused with a His tag at the C-terminus. |
| source | HEK293 |
| predicted n terminal | Arg |
| molecular weight | The recombinant 2019-nCoV Spike Protein (RBD, His Tag) consists of 234 a.a and predicts a molecular weight of 23.54 kDa. |
| purity | > 95% as determined by SDS-PAGE. |
| endotoxin | <1.0 EU per μg protein as determined by the LAL method. |
| bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)(BLPSN-0999P),the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) is 500-900ng/mL. |
| formulation | Lyophilized from sterile PBS, PH 7.4 |
| stability | The 2019-nCoV Spike Protein (RBD, His Tag) is stable for up to 1 year from date of receipt at -20℃ to -80℃. |
| usage | For Research Use Only |
| storage | Store the 2019-nCoV Spike Protein (RBD, His Tag) under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
