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| tag | His |
| host species | 2019-nCoV |
| accession | QHD43416.1 |
| synonym | S protein RBD; 2019-nCoV S protein RBD |
| background | 2019 Novel Coronavirus (2019-nCoV) is a virus (more specifically, a coronavirus) identified as the cause of an outbreak of respiratory illness first detected in Wuhan, China. |
| description | A DNA sequence encoding the 2019-nCoV Spike Protein (RBD) was expressed in Baculovirus-Insect Cells and fused with a His tag at the C-terminus. |
| source | Baculovirus-Insect Cells |
| predicted n terminal | Arg |
| molecular weight | The recombinant 2019-nCoV Spike Protein (RBD, His Tag) consists of 234 a.a and predicts a molecular weight of 26.54 kDa. |
| purity | > 90 % as determined by SDS-PAGE. |
| endotoxin | <1.0 EU per μg protein as determined by the LAL method. |
| bioactivity | The 2019-nCoV Spike Protein (RBD, His Tag) can bind with Human ACE2 in functional ELISA assay. |
| formulation | Lyophilized from sterile 20mM PB,300mM NaCl, pH 7.0,10% glycerol |
| stability | The 2019-nCoV Spike Protein (RBD, His Tag) is stable for up to 1 year from date of receipt at -20℃ to -80℃. |
| usage | For Research Use Only |
| storage | Store the 2019-nCoV Spike Protein (RBD, His Tag) under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
