Recombinant 2019-nCoV NSP10 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-10010P

Recombinant 2019-nCoV NSP10 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-10010P
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Product Overview

Tag His
Host Species 2019-nCoV
Accession YP_009725306.1
Synonym SARS-CoV 2 nsp10; SARS-CoV 2 Growth factor-like peptide; SARS-CoV 2 GFL
Background Nsp10 have shown that it is a 15-kDa protein of unknown function that has been shown to interact with itself, nsp1, and nsp7. It colocalizes with N to sites of viral replication and is essential for replication. It plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation. Nsp10 is a critical regulator of coronavirus RNA synthesis and may play an important role in polyprotein processing.
Description Recombinant 2019-nCoV NSP10 is produced by our E.coli expression system and the target gene encoding Ala1-Gln139 is expressed with a 6His tag at the N-terminus.
Source E.coli
AA Sequence Ala1-Gln139
Molecular Weight 17.9kDa. AP Mol Mass: 18kDa, reducing conditions.
Purity >80% as determined by reducing SDS-PAGE.
Formulation Recombinant 2019-nCoV NSP10 was Supplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.
Sensitivity For Research Use Only
Detection Limit Store at < -20°C, stable for 6 months after receipt. Please Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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